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Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts

Author

Listed:
  • Emil D. Jensen

    (Technical University of Denmark, Kgs)

  • Marcus Deichmann

    (Technical University of Denmark, Kgs)

  • Xin Ma

    (Technical University of Denmark, Kgs)

  • Rikke U. Vilandt

    (Technical University of Denmark, Kgs)

  • Giovanni Schiesaro

    (Technical University of Denmark, Kgs)

  • Marie B. Rojek

    (Technical University of Denmark, Kgs)

  • Bettina Lengger

    (Technical University of Denmark, Kgs)

  • Line Eliasson

    (Technical University of Denmark, Kgs)

  • Justin M. Vento

    (North Carolina State University)

  • Deniz Durmusoglu

    (North Carolina State University)

  • Sandie P. Hovmand

    (Technical University of Denmark, Kgs)

  • Ibrahim Al’Abri

    (North Carolina State University)

  • Jie Zhang

    (Technical University of Denmark, Kgs)

  • Nathan Crook

    (North Carolina State University)

  • Michael K. Jensen

    (Technical University of Denmark, Kgs)

Abstract

G protein-coupled receptors (GPCRs) enable cells to sense environmental cues and are indispensable for coordinating vital processes including quorum sensing, proliferation, and sexual reproduction. GPCRs comprise the largest class of cell surface receptors in eukaryotes, and for more than three decades the pheromone-induced mating pathway in baker’s yeast Saccharomyces cerevisiae has served as a model for studying heterologous GPCRs (hGPCRs). Here we report transcriptome profiles following mating pathway activation in native and hGPCR-signaling yeast and use a model-guided approach to correlate gene expression to morphological changes. From this we demonstrate mating between haploid cells armed with hGPCRs and endogenous biosynthesis of their cognate ligands. Furthermore, we devise a ligand-free screening strategy for hGPCR compatibility with the yeast mating pathway and enable hGPCR-signaling in the probiotic yeast Saccharomyces boulardii. Combined, our findings enable new means to study mating, hGPCR-signaling, and cell-cell communication in a model eukaryote and yeast probiotics.

Suggested Citation

  • Emil D. Jensen & Marcus Deichmann & Xin Ma & Rikke U. Vilandt & Giovanni Schiesaro & Marie B. Rojek & Bettina Lengger & Line Eliasson & Justin M. Vento & Deniz Durmusoglu & Sandie P. Hovmand & Ibrahim, 2022. "Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33961-y
    DOI: 10.1038/s41467-022-33961-y
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    References listed on IDEAS

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    1. Sonja Billerbeck & James Brisbois & Neta Agmon & Miguel Jimenez & Jasmine Temple & Michael Shen & Jef D. Boeke & Virginia W. Cornish, 2018. "A scalable peptide-GPCR language for engineering multicellular communication," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
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