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Cell type-specific calcium imaging of central sensitization in mouse dorsal horn

Author

Listed:
  • Charles Warwick

    (University of Pittsburgh)

  • Joseph Salsovic

    (University of Pittsburgh)

  • Junichi Hachisuka

    (University of Pittsburgh
    University of Glasgow)

  • Kelly M. Smith

    (University of Pittsburgh)

  • Tayler D. Sheahan

    (University of Pittsburgh)

  • Haichao Chen

    (University of Pittsburgh
    Tsinghua University)

  • James Ibinson

    (University of Pittsburgh)

  • H. Richard Koerber

    (University of Pittsburgh)

  • Sarah E. Ross

    (University of Pittsburgh
    University of Pittsburgh)

Abstract

Allodynia is a state in which pain is elicited by innocuous stimuli. Capsaicin applied to the skin results in an allodynia that extends to a broad region beyond the application site. This sensitization is thought to be mediated by spinal networks; however, we do not have a clear picture of which spinal neurons mediate this phenomenon. To address this gap, we used two-photon calcium imaging of excitatory interneurons and spinal projection neurons in the mouse spinal dorsal horn. To distinguish among neuronal subtypes, we developed CICADA, a cell profiling approach to identify cell types during calcium imaging. We then identified capsaicin-responsive and capsaicin-sensitized neuronal populations. Capsaicin-sensitized neurons showed emergent responses to innocuous input and increased receptive field sizes consistent with psychophysical reports. Finally, we identified spinal output neurons that showed enhanced responses from innocuous input. These experiments provide a population-level view of central sensitization and a framework with which to model somatosensory integration in the dorsal horn.

Suggested Citation

  • Charles Warwick & Joseph Salsovic & Junichi Hachisuka & Kelly M. Smith & Tayler D. Sheahan & Haichao Chen & James Ibinson & H. Richard Koerber & Sarah E. Ross, 2022. "Cell type-specific calcium imaging of central sensitization in mouse dorsal horn," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32608-2
    DOI: 10.1038/s41467-022-32608-2
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    References listed on IDEAS

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    1. Daniel E. Russ & Ryan B. Patterson Cross & Li Li & Stephanie C. Koch & Kaya J. E. Matson & Archana Yadav & Mor R. Alkaslasi & Dylan I. Lee & Claire E. Le Pichon & Vilas Menon & Ariel J. Levine, 2021. "A harmonized atlas of mouse spinal cord cell types and their spatial organization," Nature Communications, Nature, vol. 12(1), pages 1-20, December.
    2. Sophie Laffray & Stéphane Pagès & Hugues Dufour & Paul De Koninck & Yves De Koninck & Daniel Côté, 2011. "Adaptive Movement Compensation for In Vivo Imaging of Fast Cellular Dynamics within a Moving Tissue," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-8, May.
    3. Luana Micallef & Peter Rodgers, 2014. "eulerAPE: Drawing Area-Proportional 3-Venn Diagrams Using Ellipses," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-18, July.
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