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Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA

Author

Listed:
  • Seungha Hwang

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Paul Dominic B. Olinares

    (The Rockefeller University)

  • Jimin Lee

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Jinwoo Kim

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Brian T. Chait

    (The Rockefeller University)

  • Rodney A. King

    (Western Kentucky University)

  • Jin Young Kang

    (Korea Advanced Institute of Science and Technology (KAIST))

Abstract

Transcription, in which RNA polymerases (RNAPs) produce RNA from DNA, is the first step of gene expression. As such, it is highly regulated either by trans-elements like protein factors and/or by cis-elements like specific sequences on the DNA. Lambdoid phage HK022 contains a cis-element, put, which suppresses pausing and termination during transcription of the early phage genes. The putRNA transcript solely performs the anti-pausing/termination activities by interacting directly with the E.coli RNAP elongation complex (EC) by an unknown structural mechanism. In this study, we reconstituted putRNA-associated ECs and determined the structures using cryo-electron microscopy. The determined structures of putRNA-associated EC, putRNA-absent EC, and σ70-bound EC suggest that the putRNA interaction with the EC counteracts swiveling, a conformational change previously identified to promote pausing and σ70 might modulate putRNA folding via σ70-dependent pausing during elongation.

Suggested Citation

  • Seungha Hwang & Paul Dominic B. Olinares & Jimin Lee & Jinwoo Kim & Brian T. Chait & Rodney A. King & Jin Young Kang, 2022. "Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32315-y
    DOI: 10.1038/s41467-022-32315-y
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    Cited by:

    1. Daniela Gjorgjevikj & Naveen Kumar & Bing Wang & Tarek Hilal & Nelly Said & Bernhard Loll & Irina Artsimovitch & Ranjan Sen & Markus C. Wahl, 2025. "The Psu protein of phage satellite P4 inhibits transcription termination factor ρ by forced hyper-oligomerization," Nature Communications, Nature, vol. 16(1), pages 1-20, December.

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