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Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum

Author

Listed:
  • Céline Foulquier

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Antoine Rivière

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Mathieu Heulot

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Suzanna Reis

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Caroline Perdu

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Laurence Girbal

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Mailys Pinault

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Simon Dusséaux

    (Université de Toulouse, CNRS, INRAE, INSA)

  • Minyeong Yoo

    (Université de Toulouse, CNRS, INRAE, INSA
    University of Nottingham)

  • Philippe Soucaille

    (Université de Toulouse, CNRS, INRAE, INSA
    University of Nottingham)

  • Isabelle Meynial-Salles

    (Université de Toulouse, CNRS, INRAE, INSA)

Abstract

Clostridium acetobutylicum is a promising biocatalyst for the renewable production of n-butanol. Several metabolic strategies have already been developed to increase butanol yields, most often based on carbon pathway redirection. However, it has previously demonstrated that the activities of both ferredoxin-NADP+ reductase and ferredoxin-NAD+ reductase, whose encoding genes remain unknown, are necessary to produce the NADPH and the extra NADH needed for butanol synthesis under solventogenic conditions. Here, we purify, identify and partially characterize the proteins responsible for both activities and demonstrate the involvement of the identified enzymes in butanol synthesis through a reverse genetic approach. We further demonstrate the yield of butanol formation is limited by the level of expression of CA_C0764, the ferredoxin-NADP+ reductase encoding gene and the bcd operon, encoding a ferredoxin-NAD+ reductase. The integration of these enzymes into metabolic engineering strategies introduces opportunities for developing a homobutanologenic C. acetobutylicum strain.

Suggested Citation

  • Céline Foulquier & Antoine Rivière & Mathieu Heulot & Suzanna Reis & Caroline Perdu & Laurence Girbal & Mailys Pinault & Simon Dusséaux & Minyeong Yoo & Philippe Soucaille & Isabelle Meynial-Salles, 2022. "Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32269-1
    DOI: 10.1038/s41467-022-32269-1
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    References listed on IDEAS

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    1. Ngoc-Phuong-Thao Nguyen & Céline Raynaud & Isabelle Meynial-Salles & Philippe Soucaille, 2018. "Reviving the Weizmann process for commercial n-butanol production," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
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    Cited by:

    1. Mao, Bifei & Li, Guanghao & Wang, Mingmei & Deng, Xiangyuan & Gao, Kun & Zhang, Bingcong, 2024. "Using nitrogen starvation and excess phosphorus for two-stage algae cultivation to improve butanol production of lipid-extracted algae," Renewable Energy, Elsevier, vol. 220(C).

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