IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-31751-0.html
   My bibliography  Save this article

Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding

Author

Listed:
  • Liang Wang

    (Jiangsu Normal University)

  • Xin Wen

    (Jiangsu Normal University)

  • Zhengmao Wang

    (Qingdao National Laboratory for Marine Science and Technology
    Tsinghua University)

  • Zaisheng Lin

    (Shanghai Jiao Tong University School of Medicine)

  • Chunhong Li

    (Jiangsu Normal University)

  • Huilin Zhou

    (Jiangsu Normal University)

  • Huimin Yu

    (Jiangsu Normal University)

  • Yuhan Li

    (Jiangsu Normal University)

  • Yifei Cheng

    (Jiangsu Normal University)

  • Yuling Chen

    (Tsinghua University)

  • Geer Lou

    (Shanghai Biotree Biotech Co. Ltd)

  • Junmin Pan

    (Qingdao National Laboratory for Marine Science and Technology
    Tsinghua University)

  • Muqing Cao

    (Shanghai Jiao Tong University School of Medicine)

Abstract

The transition zone (TZ) of the cilium/flagellum serves as a diffusion barrier that controls the entry/exit of ciliary proteins. Mutations of the TZ proteins disrupt barrier function and lead to multiple human diseases. However, the systematic regulation of ciliary composition and signaling-related processes by different TZ proteins is not completely understood. Here, we reveal that loss of TCTN1 in Chlamydomonas reinhardtii disrupts the assembly of wedge-shaped structures in the TZ. Proteomic analysis of cilia from WT and three TZ mutants, tctn1, cep290, and nphp4, shows a unique role of each TZ subunit in the regulation of ciliary composition, explaining the phenotypic diversity of different TZ mutants. Interestingly, we find that defects in the TZ impair the formation and biological activity of ciliary ectosomes. Collectively, our findings provide systematic insights into the regulation of ciliary composition by TZ proteins and reveal a link between the TZ and ciliary ectosomes.

Suggested Citation

  • Liang Wang & Xin Wen & Zhengmao Wang & Zaisheng Lin & Chunhong Li & Huilin Zhou & Huimin Yu & Yuhan Li & Yifei Cheng & Yuling Chen & Geer Lou & Junmin Pan & Muqing Cao, 2022. "Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31751-0
    DOI: 10.1038/s41467-022-31751-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-31751-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-31751-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hanh M. Truong & Kevin O. Cruz-Colón & Jorge Y. Martínez-Márquez & Jason R. Willer & Amanda M. Travis & Sondip K. Biswas & Woo-Kuen Lo & Hanno J. Bolz & Jillian N. Pearring, 2023. "The tectonic complex regulates membrane protein composition in the photoreceptor cilium," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31751-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.