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Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imaging

Author

Listed:
  • Yuechuan Lin

    (Cornell University
    Massachusetts Institute of Technology)

  • Nichaluk Leartprapun

    (Cornell University
    Harvard Medical School)

  • Justin C. Luo

    (Cornell University)

  • Steven G. Adie

    (Cornell University)

Abstract

Quantitative characterisation of micro-scale mechanical properties of the extracellular matrix (ECM) and dynamic cell-ECM interactions can significantly enhance fundamental discoveries and their translational potential in the rapidly growing field of mechanobiology. However, quantitative 3D imaging of ECM mechanics with cellular-scale resolution and dynamic monitoring of cell-mediated changes to pericellular viscoelasticity remain a challenge for existing mechanical characterisation methods. Here, we present light-sheet photonic force optical coherence elastography (LS-pfOCE) to address this need by leveraging a light-sheet for parallelised, non-invasive, and localised mechanical loading. We demonstrate the capabilities of LS-pfOCE by imaging the micromechanical heterogeneity of fibrous collagen matrices and perform live-cell imaging of cell-mediated ECM micromechanical dynamics. By providing access to 4D spatiotemporal variations in the micromechanical properties of 3D biopolymer constructs and engineered cellular systems, LS-pfOCE has the potential to drive new discoveries in mechanobiology and contribute to the development of novel biomechanics-based clinical diagnostics and therapies.

Suggested Citation

  • Yuechuan Lin & Nichaluk Leartprapun & Justin C. Luo & Steven G. Adie, 2022. "Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imaging," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30995-0
    DOI: 10.1038/s41467-022-30995-0
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    References listed on IDEAS

    as
    1. Andrew D. Doyle & Nicole Carvajal & Albert Jin & Kazue Matsumoto & Kenneth M. Yamada, 2015. "Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions," Nature Communications, Nature, vol. 6(1), pages 1-15, December.
    2. Maxwell A Kotlarchyk & Samir G Shreim & Martha B Alvarez-Elizondo & Laura C Estrada & Rahul Singh & Lorenzo Valdevit & Ekaterina Kniazeva & Enrico Gratton & Andrew J Putnam & Elliot L Botvinick, 2011. "Concentration Independent Modulation of Local Micromechanics in a Fibrin Gel," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-12, May.
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