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Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity

Author

Listed:
  • Amitesh Anand

    (University of California, San Diego
    Tata Institute of Fundamental Research)

  • Arjun Patel

    (University of California, San Diego)

  • Ke Chen

    (University of California, San Diego)

  • Connor A. Olson

    (University of California, San Diego)

  • Patrick V. Phaneuf

    (University of California, San Diego)

  • Cameron Lamoureux

    (University of California, San Diego)

  • Ying Hefner

    (University of California, San Diego)

  • Richard Szubin

    (University of California, San Diego)

  • Adam M. Feist

    (University of California, San Diego
    Technical University of Denmark, Kemitorvet)

  • Bernhard O. Palsson

    (University of California, San Diego
    Technical University of Denmark, Kemitorvet)

Abstract

The bacterial respiratory electron transport system (ETS) is branched to allow condition-specific modulation of energy metabolism. There is a detailed understanding of the structural and biochemical features of respiratory enzymes; however, a holistic examination of the system and its plasticity is lacking. Here we generate four strains of Escherichia coli harboring unbranched ETS that pump 1, 2, 3, or 4 proton(s) per electron and characterized them using a combination of synergistic methods (adaptive laboratory evolution, multi-omic analyses, and computation of proteome allocation). We report that: (a) all four ETS variants evolve to a similar optimized growth rate, and (b) the laboratory evolutions generate specific rewiring of major energy-generating pathways, coupled to the ETS, to optimize ATP production capability. We thus define an Aero-Type System (ATS), which is a generalization of the aerobic bioenergetics and is a metabolic systems biology description of respiration and its inherent plasticity.

Suggested Citation

  • Amitesh Anand & Arjun Patel & Ke Chen & Connor A. Olson & Patrick V. Phaneuf & Cameron Lamoureux & Ying Hefner & Richard Szubin & Adam M. Feist & Bernhard O. Palsson, 2022. "Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30877-5
    DOI: 10.1038/s41467-022-30877-5
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    Cited by:

    1. Arjun Patel & Dominic McGrosso & Ying Hefner & Anaamika Campeau & Anand V. Sastry & Svetlana Maurya & Kevin Rychel & David J. Gonzalez & Bernhard O. Palsson, 2024. "Proteome allocation is linked to transcriptional regulation through a modularized transcriptome," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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