IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30355-y.html
   My bibliography  Save this article

PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction

Author

Listed:
  • Behnam Enghiad

    (University of Illinois Urbana-Champaign
    University of Illinois Urbana-Champaign)

  • Pu Xue

    (University of Illinois Urbana-Champaign
    University of Illinois Urbana-Champaign)

  • Nilmani Singh

    (University of Illinois Urbana-Champaign)

  • Aashutosh Girish Boob

    (University of Illinois Urbana-Champaign
    University of Illinois Urbana-Champaign)

  • Chengyou Shi

    (University of Illinois Urbana-Champaign
    University of Illinois Urbana-Champaign)

  • Vassily Andrew Petrov

    (University of Illinois Urbana-Champaign)

  • Roy Liu

    (University of Illinois Urbana-Champaign)

  • Siddhartha Suryanarayana Peri

    (University of Illinois Urbana-Champaign)

  • Stephan Thomas Lane

    (University of Illinois Urbana-Champaign)

  • Emily Danielle Gaither

    (University of Illinois Urbana-Champaign)

  • Huimin Zhao

    (University of Illinois Urbana-Champaign
    University of Illinois Urbana-Champaign)

Abstract

Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in performing sophisticated biological experiments. Here, we report the development of a versatile, robust, automated end-to-end platform named PlasmidMaker that allows error-free construction of plasmids with virtually any sequences in a high throughput manner. This platform consists of a most versatile DNA assembly method using Pyrococcus furiosus Argonaute (PfAgo)-based artificial restriction enzymes, a user-friendly frontend for plasmid design, and a backend that streamlines the workflow and integration with a robotic system. As a proof of concept, we used this platform to generate 101 plasmids from six different species ranging from 5 to 18 kb in size from up to 11 DNA fragments. PlasmidMaker should greatly expand the potential of synthetic biology.

Suggested Citation

  • Behnam Enghiad & Pu Xue & Nilmani Singh & Aashutosh Girish Boob & Chengyou Shi & Vassily Andrew Petrov & Roy Liu & Siddhartha Suryanarayana Peri & Stephan Thomas Lane & Emily Danielle Gaither & Huimin, 2022. "PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30355-y
    DOI: 10.1038/s41467-022-30355-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30355-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30355-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Siwei Li & Jingjing An & Yaqiu Li & Xiagu Zhu & Dongdong Zhao & Lixian Wang & Yonghui Sun & Yuanzhao Yang & Changhao Bi & Xueli Zhang & Meng Wang, 2022. "Automated high-throughput genome editing platform with an AI learning in situ prediction model," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30355-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.