IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30271-1.html
   My bibliography  Save this article

Fibronectin binding protein B binds to loricrin and promotes corneocyte adhesion by Staphylococcus aureus

Author

Listed:
  • Thaina M. Costa

    (School of Genetics and Microbiology, Trinity College Dublin)

  • Albertus Viljoen

    (Louvain Institute of Biomolecular Science and Technology, UC Louvain)

  • Aisling M. Towell

    (School of Genetics and Microbiology, Trinity College Dublin)

  • Yves F. Dufrêne

    (Louvain Institute of Biomolecular Science and Technology, UC Louvain
    Walloon Excellence in Life sciences and Biotechnology (WELBIO))

  • Joan A. Geoghegan

    (School of Genetics and Microbiology, Trinity College Dublin
    Institute of Microbiology and Infection, University of Birmingham, Edgbaston)

Abstract

Colonisation of humans by Staphylococcus aureus is a major risk factor for infection, yet the bacterial and host factors involved are not fully understood. The first step during skin colonisation is adhesion of the bacteria to corneocytes in the stratum corneum where the cornified envelope protein loricrin is the main ligand for S. aureus. Here we report a novel loricrin-binding protein of S. aureus, the cell wall-anchored fibronectin binding protein B (FnBPB). Single-molecule force spectroscopy revealed both weak and ultra-strong (2 nN) binding of FnBPB to loricrin and that mechanical stress enhanced the strength of these bonds. Treatment with a peptide derived from fibrinogen decreased the frequency of strong interactions, suggesting that both ligands bind to overlapping sites within FnBPB. Finally, we show that FnBPB promotes adhesion to human corneocytes by binding strongly to loricrin, highlighting the relevance of this interaction to skin colonisation.

Suggested Citation

  • Thaina M. Costa & Albertus Viljoen & Aisling M. Towell & Yves F. Dufrêne & Joan A. Geoghegan, 2022. "Fibronectin binding protein B binds to loricrin and promotes corneocyte adhesion by Staphylococcus aureus," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30271-1
    DOI: 10.1038/s41467-022-30271-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30271-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30271-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Francesc Coll & Beth Blane & Katherine L. Bellis & Marta Matuszewska & Toska Wonfor & Dorota Jamrozy & Michelle S. Toleman & Joan A. Geoghegan & Julian Parkhill & Ruth C. Massey & Sharon J. Peacock & , 2025. "The mutational landscape of Staphylococcus aureus during colonisation," Nature Communications, Nature, vol. 16(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30271-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.