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Spindle motility skews division site determination during asymmetric cell division in Physcomitrella

Author

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  • Elena Kozgunova

    (Plant Biotechnology, Faculty of Biology, University of Freiburg
    Institute for Advanced Research, Nagoya University
    Graduate School of Science, Nagoya University)

  • Mari W. Yoshida

    (Graduate School of Science, Nagoya University)

  • Ralf Reski

    (Plant Biotechnology, Faculty of Biology, University of Freiburg
    CIBSS – Centre for Integrative Biological Signalling Studies, University of Freiburg
    Cluster of Excellence livMatS @ FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg)

  • Gohta Goshima

    (Graduate School of Science, Nagoya University
    Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University)

Abstract

Asymmetric cell division (ACD) underlies the development of multicellular organisms. In animal ACD, the cell division site is determined by active spindle-positioning mechanisms. In contrast, it is considered that the division site in plants is determined prior to mitosis by the microtubule-actin belt known as the preprophase band (PPB) and that the localization of the mitotic spindle is typically static and does not govern the division plane. However, in some plant species, ACD occurs in the absence of PPB. Here, we isolate a hypomorphic mutant of the conserved microtubule-associated protein TPX2 in the moss Physcomitrium patens (Physcomitrella) and observe spindle motility during PPB-independent cell division. This defect compromises the position of the division site and produces inverted daughter cell sizes in the first ACD of gametophore (leafy shoot) development. The phenotype is rescued by restoring endogenous TPX2 function and, unexpectedly, by depolymerizing actin filaments. Thus, we identify an active spindle-positioning mechanism that, reminiscent of acentrosomal ACD in animals, involves microtubules and actin filaments, and sets the division site in plants.

Suggested Citation

  • Elena Kozgunova & Mari W. Yoshida & Ralf Reski & Gohta Goshima, 2022. "Spindle motility skews division site determination during asymmetric cell division in Physcomitrella," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30239-1
    DOI: 10.1038/s41467-022-30239-1
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    References listed on IDEAS

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    1. Xing Duan & Yizeng Li & Kexi Yi & Fengli Guo & HaiYang Wang & Pei-Hsun Wu & Jing Yang & Devin B. Mair & Edwin Angelo Morales & Petr Kalab & Denis Wirtz & Sean X. Sun & Rong Li, 2020. "Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
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