Author
Listed:
- Chengjin Zhu
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Xieyang Guo
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Philippe Dumas
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Maria Takacs
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Mo’men Abdelkareem
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Arnaud Vanden Broeck
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Charlotte Saint-André
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Gabor Papai
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Corinne Crucifix
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Julio Ortiz
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
- Albert Weixlbaumer
(Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
Université de Strasbourg
Centre National de la Recherche Scientifique, UMR7104
Institut National de la Santé et de la Recherche Médicale, U1258)
Abstract
RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind RNAP simultaneously. Here we report cryo-EM reconstructions of Escherichia coli RNAP bound to NusG, or NusA, or both. RNAP conformational changes, referred to as swivelling, correlate with transcriptional pausing. NusA facilitates RNAP swivelling to further increase pausing, while NusG counteracts this role. Their structural effects are consistent with biochemical results on two categories of transcriptional pauses. In addition, the structures suggest a cooperative mechanism of NusA and NusG during Rho-mediated transcription termination. Our results provide a structural rationale for the stochastic nature of pausing and termination and how NusA and NusG can modulate it.
Suggested Citation
Chengjin Zhu & Xieyang Guo & Philippe Dumas & Maria Takacs & Mo’men Abdelkareem & Arnaud Vanden Broeck & Charlotte Saint-André & Gabor Papai & Corinne Crucifix & Julio Ortiz & Albert Weixlbaumer, 2022.
"Transcription factors modulate RNA polymerase conformational equilibrium,"
Nature Communications, Nature, vol. 13(1), pages 1-12, December.
Handle:
RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29148-0
DOI: 10.1038/s41467-022-29148-0
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Citations
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Cited by:
- Adrien Chauvier & Shiba S. Dandpat & Rosa Romero & Nils G. Walter, 2024.
"A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integration,"
Nature Communications, Nature, vol. 15(1), pages 1-18, December.
- Simona Pilotto & Michal Sýkora & Gwenny Cackett & Christopher Dulson & Finn Werner, 2024.
"Structure of the recombinant RNA polymerase from African Swine Fever Virus,"
Nature Communications, Nature, vol. 15(1), pages 1-15, December.
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