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FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity

Author

Listed:
  • Zifeng Cui

    (Sun Yat-sen University)

  • Rui Tian

    (Sun Yat-sen University, Guangzhou
    Sun Yat-sen University Nanchang Research Institution)

  • Zhaoyue Huang

    (Sun Yat-sen University)

  • Zhuang Jin

    (Sun Yat-sen University)

  • Lifang Li

    (Sun Yat-sen University)

  • Jiashuo Liu

    (Sun Yat-sen University)

  • Zheying Huang

    (Sun Yat-sen University)

  • Hongxian Xie

    (Generulor Company Bio-X Lab)

  • Dan Liu

    (Generulor Company Bio-X Lab)

  • Haiyan Mo

    (Generulor Company Bio-X Lab)

  • Rong Zhou

    (Generulor Company Bio-X Lab)

  • Bin Lang

    (Macao Polytechnic Institute)

  • Bo Meng

    (University of Science and Technology of China)

  • Haiyan Weng

    (University of Science and Technology of China)

  • Zheng Hu

    (Sun Yat-sen University
    Sun Yat-sen University Nanchang Research Institution)

Abstract

Genome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene editing tools with superior cutting efficiency, good fidelity, and fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium, which is characterized by a simple PAM (5′-NNTA-3′) and a guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency and specificity to SpCas9. Interestingly, the PAM of FrCas9 presents a palindromic sequence, which greatly expands its targeting scope. Due to the PAM sequence, FrCas9 possesses double editing-windows for base editor and could directly target the TATA-box in eukaryotic promoters for TATA-box related diseases. Together, our results broaden the understanding of CRISPR/Cas-mediated genome engineering and establish FrCas9 as a safe and efficient platform for wide applications in research, biotechnology and therapeutics.

Suggested Citation

  • Zifeng Cui & Rui Tian & Zhaoyue Huang & Zhuang Jin & Lifang Li & Jiashuo Liu & Zheying Huang & Hongxian Xie & Dan Liu & Haiyan Mo & Rong Zhou & Bin Lang & Bo Meng & Haiyan Weng & Zheng Hu, 2022. "FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29089-8
    DOI: 10.1038/s41467-022-29089-8
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    Cited by:

    1. Zhaohui Zhong & Guanqing Liu & Zhongjie Tang & Shuyue Xiang & Liang Yang & Lan Huang & Yao He & Tingting Fan & Shishi Liu & Xuelian Zheng & Tao Zhang & Yiping Qi & Jian Huang & Yong Zhang, 2023. "Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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