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PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides

Author

Listed:
  • Jeffrey S. Rush

    (University of Kentucky)

  • Prakash Parajuli

    (University of Kentucky)

  • Alessandro Ruda

    (Stockholm University)

  • Jian Li

    (University of Kentucky)

  • Amol Arunrao Pohane

    (University of Kentucky)

  • Svetlana Zamakhaeva

    (University of Kentucky)

  • Mohammad M. Rahman

    (University of Kentucky)

  • Jennifer C. Chang

    (University of Illinois at Chicago)

  • Artemis Gogos

    (University of Illinois at Chicago)

  • Cameron W. Kenner

    (University of Kentucky)

  • Gérard Lambeau

    (Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire)

  • Michael J. Federle

    (University of Illinois at Chicago)

  • Konstantin V. Korotkov

    (University of Kentucky)

  • Göran Widmalm

    (Stockholm University)

  • Natalia Korotkova

    (University of Kentucky
    University of Kentucky)

Abstract

The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists of peptidoglycan decorated with the Lancefield group A carbohydrate (GAC). GAC is a promising target for the development of GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that GAC is attached to peptidoglycan via glucosamine 1-phosphate. This structural feature makes the GAC-peptidoglycan linkage highly sensitive to cleavage by nitrous acid and resistant to mild acid conditions. Using this characteristic of the GAS cell wall, we identify PplD as a protein required for deacetylation of linkage N-acetylglucosamine (GlcNAc). X-ray structural analysis indicates that PplD performs catalysis via a modified acid/base mechanism. Genetic surveys in silico together with functional analysis indicate that PplD homologs deacetylate the polysaccharide linkage in many streptococcal species. We further demonstrate that introduction of positive charges to the cell wall by GlcNAc deacetylation protects GAS against host cationic antimicrobial proteins.

Suggested Citation

  • Jeffrey S. Rush & Prakash Parajuli & Alessandro Ruda & Jian Li & Amol Arunrao Pohane & Svetlana Zamakhaeva & Mohammad M. Rahman & Jennifer C. Chang & Artemis Gogos & Cameron W. Kenner & Gérard Lambeau, 2022. "PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28257-0
    DOI: 10.1038/s41467-022-28257-0
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