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Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions

Author

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  • Patrycja Stachelek

    (Durham University)

  • Lewis MacKenzie

    (Durham University)

  • David Parker

    (Durham University)

  • Robert Pal

    (Durham University)

Abstract

The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary imaging community, allowing the study of chiral interactions on a sub-cellular level in a new (chiral) light.

Suggested Citation

  • Patrycja Stachelek & Lewis MacKenzie & David Parker & Robert Pal, 2022. "Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28220-z
    DOI: 10.1038/s41467-022-28220-z
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    Cited by:

    1. Qi Liu & Qi Wei & Hui Ren & Luwei Zhou & Yifan Zhou & Pengzhi Wang & Chenghao Wang & Jun Yin & Mingjie Li, 2023. "Circular polarization-resolved ultraviolet photonic artificial synapse based on chiral perovskite," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Davide F. Rosa & Patrycja Stachelek & Dominic J. Black & Robert Pal, 2023. "Rapid handheld time-resolved circularly polarised luminescence photography camera for life and material sciences," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Jana Bocková & Nykola C. Jones & Jérémie Topin & Søren V. Hoffmann & Cornelia Meinert, 2023. "Uncovering the chiral bias of meteoritic isovaline through asymmetric photochemistry," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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