IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27489-w.html
   My bibliography  Save this article

Oxicam-type non-steroidal anti-inflammatory drugs inhibit NPR1-mediated salicylic acid pathway

Author

Listed:
  • Nobuaki Ishihama

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science)

  • Seung-won Choi

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science)

  • Yoshiteru Noutoshi

    (Okayama University)

  • Ivana Saska

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science)

  • Shuta Asai

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science)

  • Kaori Takizawa

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science)

  • Sheng Yang He

    (Michigan State University
    Michigan State University)

  • Hiroyuki Osada

    (Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science)

  • Ken Shirasu

    (Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science
    The University of Tokyo)

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs), including salicylic acid (SA), target mammalian cyclooxygenases. In plants, SA is a defense hormone that regulates NON-EXPRESSOR OF PATHOGENESIS RELATED GENES 1 (NPR1), the master transcriptional regulator of immunity-related genes. We identify that the oxicam-type NSAIDs tenoxicam (TNX), meloxicam, and piroxicam, but not other types of NSAIDs, exhibit an inhibitory effect on immunity to bacteria and SA-dependent plant immune response. TNX treatment decreases NPR1 levels, independently from the proposed SA receptors NPR3 and NPR4. Instead, TNX induces oxidation of cytosolic redox status, which is also affected by SA and regulates NPR1 homeostasis. A cysteine labeling assay reveals that cysteine residues in NPR1 can be oxidized in vitro, leading to disulfide-bridged oligomerization of NPR1, but not in vivo regardless of SA or TNX treatment. Therefore, this study indicates that oxicam inhibits NPR1-mediated SA signaling without affecting the redox status of NPR1.

Suggested Citation

  • Nobuaki Ishihama & Seung-won Choi & Yoshiteru Noutoshi & Ivana Saska & Shuta Asai & Kaori Takizawa & Sheng Yang He & Hiroyuki Osada & Ken Shirasu, 2021. "Oxicam-type non-steroidal anti-inflammatory drugs inhibit NPR1-mediated salicylic acid pathway," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27489-w
    DOI: 10.1038/s41467-021-27489-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27489-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27489-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27489-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.