IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27378-2.html
   My bibliography  Save this article

Massive image-based single-cell profiling reveals high levels of circulating platelet aggregates in patients with COVID-19

Author

Listed:
  • Masako Nishikawa

    (The University of Tokyo)

  • Hiroshi Kanno

    (The University of Tokyo)

  • Yuqi Zhou

    (The University of Tokyo)

  • Ting-Hui Xiao

    (The University of Tokyo)

  • Takuma Suzuki

    (The University of Tokyo)

  • Yuma Ibayashi

    (The University of Tokyo)

  • Jeffrey Harmon

    (The University of Tokyo)

  • Shigekazu Takizawa

    (The University of Tokyo)

  • Kotaro Hiramatsu

    (The University of Tokyo
    The University of Tokyo)

  • Nao Nitta

    (CYBO, Inc)

  • Risako Kameyama

    (The University of Tokyo)

  • Walker Peterson

    (The University of Tokyo)

  • Jun Takiguchi

    (The University of Tokyo)

  • Mohammad Shifat-E-Rabbi

    (University of Virginia)

  • Yan Zhuang

    (University of Virginia)

  • Xuwang Yin

    (University of Virginia)

  • Abu Hasnat Mohammad Rubaiyat

    (University of Virginia)

  • Yunjie Deng

    (The University of Tokyo)

  • Hongqian Zhang

    (The University of Tokyo)

  • Shigeki Miyata

    (Japanese Red Cross Society)

  • Gustavo K. Rohde

    (University of Virginia
    University of Virginia)

  • Wataru Iwasaki

    (The University of Tokyo
    The University of Tokyo
    The University of Tokyo)

  • Yutaka Yatomi

    (The University of Tokyo)

  • Keisuke Goda

    (The University of Tokyo
    Wuhan University
    University of California)

Abstract

A characteristic clinical feature of COVID-19 is the frequent incidence of microvascular thrombosis. In fact, COVID-19 autopsy reports have shown widespread thrombotic microangiopathy characterized by extensive diffuse microthrombi within peripheral capillaries and arterioles in lungs, hearts, and other organs, resulting in multiorgan failure. However, the underlying process of COVID-19-associated microvascular thrombosis remains elusive due to the lack of tools to statistically examine platelet aggregation (i.e., the initiation of microthrombus formation) in detail. Here we report the landscape of circulating platelet aggregates in COVID-19 obtained by massive single-cell image-based profiling and temporal monitoring of the blood of COVID-19 patients (n = 110). Surprisingly, our analysis of the big image data shows the anomalous presence of excessive platelet aggregates in nearly 90% of all COVID-19 patients. Furthermore, results indicate strong links between the concentration of platelet aggregates and the severity, mortality, respiratory condition, and vascular endothelial dysfunction level of COVID-19 patients.

Suggested Citation

  • Masako Nishikawa & Hiroshi Kanno & Yuqi Zhou & Ting-Hui Xiao & Takuma Suzuki & Yuma Ibayashi & Jeffrey Harmon & Shigekazu Takizawa & Kotaro Hiramatsu & Nao Nitta & Risako Kameyama & Walker Peterson & , 2021. "Massive image-based single-cell profiling reveals high levels of circulating platelet aggregates in patients with COVID-19," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27378-2
    DOI: 10.1038/s41467-021-27378-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27378-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27378-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hiroshi Kanno & Kotaro Hiramatsu & Hideharu Mikami & Atsushi Nakayashiki & Shota Yamashita & Arata Nagai & Kohki Okabe & Fan Li & Fei Yin & Keita Tominaga & Omer Faruk Bicer & Ryohei Noma & Bahareh Ki, 2024. "High-throughput fluorescence lifetime imaging flow cytometry," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27378-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.