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A genetically encoded fluorescent biosensor for extracellular l-lactate

Author

Listed:
  • Yusuke Nasu

    (The University of Tokyo, Bunkyo-ku)

  • Ciaran Murphy-Royal

    (Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
    Centre Hospitalier de l’Université de Montréal, Department of Neuroscience, Faculty of Medicine, University of Montreal)

  • Yurong Wen

    (University of Alberta
    The First Affiliated Hospital, Xi’an Jiaotong University)

  • Jordan N. Haidey

    (Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary)

  • Rosana S. Molina

    (Montana State University)

  • Abhi Aggarwal

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Shuce Zhang

    (University of Alberta)

  • Yuki Kamijo

    (The University of Tokyo, Bunkyo-ku)

  • Marie-Eve Paquet

    (CERVO Brain Research Center, Laval University
    Microbiology and Bioinformatics, Laval University)

  • Kaspar Podgorski

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Mikhail Drobizhev

    (Montana State University)

  • Jaideep S. Bains

    (Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary)

  • M. Joanne Lemieux

    (University of Alberta)

  • Grant R. Gordon

    (Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary)

  • Robert E. Campbell

    (The University of Tokyo, Bunkyo-ku
    University of Alberta)

Abstract

l-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of l-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular l-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular l-lactate in cultured mammalian cells and brain tissue.

Suggested Citation

  • Yusuke Nasu & Ciaran Murphy-Royal & Yurong Wen & Jordan N. Haidey & Rosana S. Molina & Abhi Aggarwal & Shuce Zhang & Yuki Kamijo & Marie-Eve Paquet & Kaspar Podgorski & Mikhail Drobizhev & Jaideep S. , 2021. "A genetically encoded fluorescent biosensor for extracellular l-lactate," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27332-2
    DOI: 10.1038/s41467-021-27332-2
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