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A microfluidic platform for highly parallel bite by bite profiling of mosquito-borne pathogen transmission

Author

Listed:
  • Shailabh Kumar

    (Stanford University)

  • Felix J. H. Hol

    (Stanford University
    Institut Pasteur, UMR2000, CNRS
    Université de Paris)

  • Sujit Pujhari

    (The Pennsylvania State University
    University of South Carolina School of Medicine)

  • Clayton Ellington

    (Stanford University)

  • Haripriya Vaidehi Narayanan

    (Stanford University)

  • Hongquan Li

    (Stanford University)

  • Jason L. Rasgon

    (The Pennsylvania State University)

  • Manu Prakash

    (Stanford University
    Stanford University)

Abstract

Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a low-cost, scalable microfluidic platform enabling high-throughput molecular interrogation of individual mosquito bites. We introduce an ultra-thin PDMS membrane which acts as a biting interface to arrays of micro-wells. Freely-behaving mosquitoes deposit saliva droplets by biting into these micro-wells. By modulating membrane thickness, we observe species-dependent differences in mosquito biting capacity, utilizable for selective sample collection. We demonstrate RT-PCR and focus-forming assays on-chip to detect mosquito DNA, Zika virus RNA, as well as quantify infectious Mayaro virus particles transmitted from single mosquito bites. The Vectorchip presents a promising approach for single-bite-resolution laboratory and field characterization of vector-pathogen communities, and could serve as a powerful early warning sentinel for mosquito-borne diseases.

Suggested Citation

  • Shailabh Kumar & Felix J. H. Hol & Sujit Pujhari & Clayton Ellington & Haripriya Vaidehi Narayanan & Hongquan Li & Jason L. Rasgon & Manu Prakash, 2021. "A microfluidic platform for highly parallel bite by bite profiling of mosquito-borne pathogen transmission," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26300-0
    DOI: 10.1038/s41467-021-26300-0
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