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Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold

Author

Listed:
  • Nina Schweizer

    (Mechanisms of Disease Programme, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST))

  • Laurence Haren

    (Molecular, Cellular and Developmental Biology, Centre de Biologie Intégrative, CNRS-Université Toulouse III)

  • Ilaria Dutto

    (Mechanisms of Disease Programme, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST))

  • Ricardo Viais

    (Mechanisms of Disease Programme, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST))

  • Cristina Lacasa

    (Mechanisms of Disease Programme, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST))

  • Andreas Merdes

    (Molecular, Cellular and Developmental Biology, Centre de Biologie Intégrative, CNRS-Université Toulouse III)

  • Jens Lüders

    (Mechanisms of Disease Programme, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST))

Abstract

Centriole biogenesis and maintenance are crucial for cells to generate cilia and assemble centrosomes that function as microtubule organizing centers (MTOCs). Centriole biogenesis and MTOC function both require the microtubule nucleator γ-tubulin ring complex (γTuRC). It is widely accepted that γTuRC nucleates microtubules from the pericentriolar material that is associated with the proximal part of centrioles. However, γTuRC also localizes more distally and in the centriole lumen, but the significance of these findings is unclear. Here we identify spatially and functionally distinct subpopulations of centrosomal γTuRC. Luminal localization is mediated by augmin, which is linked to the centriole inner scaffold through POC5. Disruption of luminal localization impairs centriole integrity and interferes with cilium assembly. Defective ciliogenesis is also observed in γTuRC mutant fibroblasts from a patient suffering from microcephaly with chorioretinopathy. These results identify a non-canonical role of augmin-γTuRC in the centriole lumen that is linked to human disease.

Suggested Citation

  • Nina Schweizer & Laurence Haren & Ilaria Dutto & Ricardo Viais & Cristina Lacasa & Andreas Merdes & Jens Lüders, 2021. "Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26252-5
    DOI: 10.1038/s41467-021-26252-5
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    References listed on IDEAS

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    1. Peng Liu & Erik Zupa & Annett Neuner & Anna Böhler & Justus Loerke & Dirk Flemming & Thomas Ruppert & Till Rudack & Christoph Peter & Christian Spahn & Oliver J. Gruss & Stefan Pfeffer & Elmar Schiebe, 2020. "Insights into the assembly and activation of the microtubule nucleator γ-TuRC," Nature, Nature, vol. 578(7795), pages 467-471, February.
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    1. Yutaka Takeda & Takumi Chinen & Shunnosuke Honda & Sho Takatori & Shotaro Okuda & Shohei Yamamoto & Masamitsu Fukuyama & Koh Takeuchi & Taisuke Tomita & Shoji Hata & Daiju Kitagawa, 2024. "Molecular basis promoting centriole triplet microtubule assembly," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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