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Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms

Author

Listed:
  • Ho-Soo Lee

    (Ajou University School of Medicine)

  • Sunwoo Min

    (Ajou University School of Medicine)

  • Ye-Eun Jung

    (Ewha Womans University)

  • Sunyoung Chae

    (Ajou University School of Medicine)

  • June Heo

    (Ajou University School of Medicine
    Graduate School of Ajou University)

  • Jae-Ho Lee

    (Ajou University School of Medicine
    Ajou University School of Medicine)

  • TaeSoo Kim

    (Ewha Womans University)

  • Ho-Chul Kang

    (Ajou University School of Medicine)

  • Makoto Nakanish

    (The University of Tokyo)

  • Sun-Shin Cha

    (Ewha Womans University)

  • Hyeseong Cho

    (Ajou University School of Medicine)

Abstract

The chromatin remodeler RSF1 enriched at mitotic centromeres is essential for proper chromosome alignment and segregation and underlying mechanisms remain to be disclosed. We here show that PLK1 recruitment by RSF1 at centromeres creates an activating phosphorylation on Thr236 in the activation loop of Aurora B and this is indispensable for the Aurora B activation. In structural modeling the phosphorylated Thr236 enhances the base catalysis by Asp200 nearby, facilitating the Thr232 autophosphorylation. Accordingly, RSF1-PLK1 is central for Aurora B-mediated microtubule destabilization in error correction. However, under full microtubule-kinetochore attachment RSF1-PLK1 positions at kinetochores, halts activating Aurora B and phosphorylates BubR1, regardless of tension. Spatial movement of RSF1-PLK1 to kinetochores is triggered by Aurora B-mediated phosphorylation of centromeric histone H3 on Ser28. We propose a regulatory RSF1-PLK1 axis that spatiotemporally controls on/off switch on Aurora B. This feedback circuit among RSF1-PLK1-Aurora B may coordinate dynamic microtubule-kinetochore attachment in early mitosis when full tension yet to be generated.

Suggested Citation

  • Ho-Soo Lee & Sunwoo Min & Ye-Eun Jung & Sunyoung Chae & June Heo & Jae-Ho Lee & TaeSoo Kim & Ho-Chul Kang & Makoto Nakanish & Sun-Shin Cha & Hyeseong Cho, 2021. "Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26220-z
    DOI: 10.1038/s41467-021-26220-z
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    References listed on IDEAS

    as
    1. Luying Jia & Bing Li & Hongtao Yu, 2016. "The Bub1–Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    2. Ho-Soo Lee & Yong-Yea Park & Mi-Young Cho & Sunyoung Chae & Young-Suk Yoo & Myung-Hee Kwon & Chang-Woo Lee & Hyeseong Cho, 2015. "The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
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