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Structural and functional characterization of the bacterial biofilm activator RemA

Author

Listed:
  • Tamara Hoffmann

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Biology)

  • Devid Mrusek

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry)

  • Patricia Bedrunka

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry)

  • Fabiana Burchert

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry)

  • Christopher-Nils Mais

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry)

  • Daniel B. Kearns

    (Indiana University)

  • Florian Altegoer

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry)

  • Erhard Bremer

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Biology)

  • Gert Bange

    (Center for Synthetic Microbiology (SYNMIKRO) & Faculty of Chemistry
    Max Planck Institute for Terrestrial Microbiology)

Abstract

Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain.

Suggested Citation

  • Tamara Hoffmann & Devid Mrusek & Patricia Bedrunka & Fabiana Burchert & Christopher-Nils Mais & Daniel B. Kearns & Florian Altegoer & Erhard Bremer & Gert Bange, 2021. "Structural and functional characterization of the bacterial biofilm activator RemA," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26005-4
    DOI: 10.1038/s41467-021-26005-4
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