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In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli

Author

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  • Philipp S. Erdmann

    (Max Planck Institute of Biochemistry
    Fondazione Human Technopole)

  • Zhen Hou

    (Max Planck Institute of Biochemistry)

  • Sven Klumpe

    (Max Planck Institute of Biochemistry)

  • Sagar Khavnekar

    (Max Planck Institute of Biochemistry)

  • Florian Beck

    (Max Planck Institute of Biochemistry)

  • Florian Wilfling

    (Max Planck Institute of Biochemistry
    Max Planck Institute of Biophysics)

  • Jürgen M. Plitzko

    (Max Planck Institute of Biochemistry)

  • Wolfgang Baumeister

    (Max Planck Institute of Biochemistry)

Abstract

Ribosomes comprise a large (LSU) and a small subunit (SSU) which are synthesized independently in the nucleolus before being exported into the cytoplasm, where they assemble into functional ribosomes. Individual maturation steps have been analyzed in detail using biochemical methods, light microscopy and conventional electron microscopy (EM). In recent years, single particle analysis (SPA) has yielded molecular resolution structures of several pre-ribosomal intermediates. It falls short, however, of revealing the spatiotemporal sequence of ribosome biogenesis in the cellular context. Here, we present our study on native nucleoli in Chlamydomonas reinhardtii, in which we follow the formation of LSU and SSU precursors by in situ cryo-electron tomography (cryo-ET) and subtomogram averaging (STA). By combining both positional and molecular data, we reveal gradients of ribosome maturation within the granular component (GC), offering a new perspective on how the liquid-liquid-phase separation of the nucleolus supports ribosome biogenesis.

Suggested Citation

  • Philipp S. Erdmann & Zhen Hou & Sven Klumpe & Sagar Khavnekar & Florian Beck & Florian Wilfling & Jürgen M. Plitzko & Wolfgang Baumeister, 2021. "In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25413-w
    DOI: 10.1038/s41467-021-25413-w
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    Cited by:

    1. Haonan Zhang & Yan Li & Yanan Liu & Dongyu Li & Lin Wang & Kai Song & Keyan Bao & Ping Zhu, 2023. "A method for restoring signals and revealing individual macromolecule states in cryo-ET, REST," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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