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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Author

Listed:
  • Mohsen Mohammadniaei

    (Technical University of Denmark)

  • Ming Zhang

    (Technical University of Denmark)

  • Jon Ashley

    (Technical University of Denmark)

  • Ulf Bech Christensen

    (PentaBase A/S)

  • Lennart Jan Friis-Hansen

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Rasmus Gregersen

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Jan Gorm Lisby

    (Copenhagen University Hospital - Amager and Hvidovre)

  • Thomas Lars Benfield

    (Copenhagen University Hospital - Amager and Hvidovre)

  • Finn Erland Nielsen

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Jens Henning Rasmussen

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg
    Center for Translational Research, Copenhagen University Hospital – Bispebjerg and Frederiksberg)

  • Ellen Bøtker Pedersen

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Anne Christine Rye Olinger

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Lærke Tørring Kolding

    (Copenhagen University Hospital - Bispebjerg and Frederiksberg)

  • Maryam Naseri

    (Technical University of Denmark)

  • Tao Zheng

    (Technical University of Denmark)

  • Wentao Wang

    (Technical University of Denmark)

  • Jan Gorodkin

    (University of Copenhagen)

  • Yi Sun

    (Technical University of Denmark)

Abstract

The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast ( 1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

Suggested Citation

  • Mohsen Mohammadniaei & Ming Zhang & Jon Ashley & Ulf Bech Christensen & Lennart Jan Friis-Hansen & Rasmus Gregersen & Jan Gorm Lisby & Thomas Lars Benfield & Finn Erland Nielsen & Jens Henning Rasmuss, 2021. "A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25387-9
    DOI: 10.1038/s41467-021-25387-9
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