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A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Author

Listed:
  • Doyeon Kim

    (Seoul National University)

  • Sukjun Kim

    (Seoul National University)

  • Joori Park

    (Korea University
    Korea University)

  • Hee Ryung Chang

    (Seoul National University)

  • Jeeyoon Chang

    (Korea University
    Korea University)

  • Junhak Ahn

    (Seoul National University)

  • Heedo Park

    (Korea University)

  • Junehee Park

    (Seoul National University)

  • Narae Son

    (Seoul National University)

  • Gihyeon Kang

    (Seoul National University)

  • Jeonghun Kim

    (Korea University)

  • Kisoon Kim

    (Korea University)

  • Man-Seong Park

    (Korea University)

  • Yoon Ki Kim

    (Korea University
    Korea University)

  • Daehyun Baek

    (Seoul National University
    Seoul National University)

Abstract

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

Suggested Citation

  • Doyeon Kim & Sukjun Kim & Joori Park & Hee Ryung Chang & Jeeyoon Chang & Junhak Ahn & Heedo Park & Junehee Park & Narae Son & Gihyeon Kang & Jeonghun Kim & Kisoon Kim & Man-Seong Park & Yoon Ki Kim & , 2021. "A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25361-5
    DOI: 10.1038/s41467-021-25361-5
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    Cited by:

    1. Pawel M. Wydorski & Jerzy Osipiuk & Benjamin T. Lanham & Christine Tesar & Michael Endres & Elizabeth Engle & Robert Jedrzejczak & Vishruth Mullapudi & Karolina Michalska & Krzysztof Fidelis & David F, 2023. "Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Emilie Murigneux & Laurent Softic & Corentin Aubé & Carmen Grandi & Delphine Judith & Johanna Bruce & Morgane Le Gall & François Guillonneau & Alain Schmitt & Vincent Parissi & Clarisse Berlioz-Torren, 2024. "Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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