IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-25288-x.html
   My bibliography  Save this article

Systems approaches identify the consequences of monosomy in somatic human cells

Author

Listed:
  • Narendra Kumar Chunduri

    (TU Kaiserslautern)

  • Paul Menges

    (TU Kaiserslautern)

  • Xiaoxiao Zhang

    (University of Applied Sciences Koblenz)

  • Angela Wieland

    (TU Kaiserslautern)

  • Vincent Leon Gotsmann

    (TU Kaiserslautern)

  • Balca R. Mardin

    (European Molecular Biology Laboratory (EMBL))

  • Christopher Buccitelli

    (European Molecular Biology Laboratory (EMBL))

  • Jan O. Korbel

    (European Molecular Biology Laboratory (EMBL))

  • Felix Willmund

    (TU Kaiserslautern)

  • Maik Kschischo

    (University of Applied Sciences Koblenz)

  • Markus Raeschle

    (TU Kaiserslautern)

  • Zuzana Storchova

    (TU Kaiserslautern)

Abstract

Chromosome loss that results in monosomy is detrimental to viability, yet it is frequently observed in cancers. How cancers survive with monosomy is unknown. Using p53-deficient monosomic cell lines, we find that chromosome loss impairs proliferation and genomic stability. Transcriptome and proteome analysis demonstrates reduced expression of genes encoded on the monosomes, which is partially compensated in some cases. Monosomy also induces global changes in gene expression. Pathway enrichment analysis reveals that genes involved in ribosome biogenesis and translation are downregulated in all monosomic cells analyzed. Consistently, monosomies display defects in protein synthesis and ribosome assembly. We further show that monosomies are incompatible with p53 expression, likely due to defects in ribosome biogenesis. Accordingly, impaired ribosome biogenesis and p53 inactivation are associated with monosomy in cancer. Our systematic study of monosomy in human cells explains why monosomy is so detrimental and reveals the importance of p53 for monosomy occurrence in cancer.

Suggested Citation

  • Narendra Kumar Chunduri & Paul Menges & Xiaoxiao Zhang & Angela Wieland & Vincent Leon Gotsmann & Balca R. Mardin & Christopher Buccitelli & Jan O. Korbel & Felix Willmund & Maik Kschischo & Markus Ra, 2021. "Systems approaches identify the consequences of monosomy in somatic human cells," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25288-x
    DOI: 10.1038/s41467-021-25288-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-25288-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-25288-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Johanna Zerbib & Marica Rosaria Ippolito & Yonatan Eliezer & Giuseppina Feudis & Eli Reuveni & Anouk Savir Kadmon & Sara Martin & Sonia Viganò & Gil Leor & James Berstler & Julia Muenzner & Michael Mü, 2024. "Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25288-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.