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Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health

Author

Listed:
  • Thi Phuong Nam Bui

    (Wageningen University
    Caelus Pharmaceuticals)

  • Louise Mannerås-Holm

    (University of Gothenburg)

  • Robert Puschmann

    (Leibniz-Forschungsinstitut für Molekulare Pharmakologie
    Humboldt-Universität zu Berlin)

  • Hao Wu

    (University of Gothenburg
    Fudan University)

  • Antonio Dario Troise

    (Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council)

  • Bart Nijsse

    (Wageningen University)

  • Sjef Boeren

    (Wageningen University)

  • Fredrik Bäckhed

    (University of Gothenburg
    University of Copenhagen
    Sahlgrenska University Hospital, Department of Clinical Physiology)

  • Dorothea Fiedler

    (Leibniz-Forschungsinstitut für Molekulare Pharmakologie
    Humboldt-Universität zu Berlin)

  • Willem M. deVos

    (Wageningen University
    University of Helsinki)

Abstract

We describe the anaerobic conversion of inositol stereoisomers to propionate and acetate by the abundant intestinal genus Anaerostipes. A inositol pathway was elucidated by nuclear magnetic resonance using [13C]-inositols, mass spectrometry and proteogenomic analyses in A. rhamnosivorans, identifying 3-oxoacid CoA transferase as a key enzyme involved in both 3-oxopropionyl-CoA and propionate formation. This pathway also allowed conversion of phytate-derived inositol into propionate as shown with [13C]-phytate in fecal samples amended with A. rhamnosivorans. Metabolic and (meta)genomic analyses explained the adaptation of Anaerostipes spp. to inositol-containing substrates and identified a propionate-production gene cluster to be inversely associated with metabolic biomarkers in (pre)diabetes cohorts. Co-administration of myo-inositol with live A. rhamnosivorans in western-diet fed mice reduced fasting-glucose levels comparing to heat-killed A. rhamnosivorans after 6-weeks treatment. Altogether, these data suggest a potential beneficial role for intestinal Anaerostipes spp. in promoting host health.

Suggested Citation

  • Thi Phuong Nam Bui & Louise Mannerås-Holm & Robert Puschmann & Hao Wu & Antonio Dario Troise & Bart Nijsse & Sjef Boeren & Fredrik Bäckhed & Dorothea Fiedler & Willem M. deVos, 2021. "Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25081-w
    DOI: 10.1038/s41467-021-25081-w
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