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Physical basis for the determination of lumen shape in a simple epithelium

Author

Listed:
  • Claudia G. Vasquez

    (Department of Chemical Engineering)

  • Vipul T. Vachharajani

    (Stanford University)

  • Carlos Garzon-Coral

    (Department of Chemical Engineering)

  • Alexander R. Dunn

    (Stanford University School of Medicine)

Abstract

The formation of a hollow lumen in a formerly solid mass of cells is a key developmental process whose dysregulation leads to diseases of the kidney and other organs. Hydrostatic pressure has been proposed to drive lumen expansion, a view that is supported by experiments in the mouse blastocyst. However, lumens formed in other tissues adopt irregular shapes with cell apical faces that are bowed inward, suggesting that pressure may not be the dominant contributor to lumen shape in all cases. Here we use live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney cell spheroids, a canonical cell-culture model for lumenogenesis. We find that in this system, lumen shape reflects basic geometrical considerations tied to the establishment of apico-basal polarity. A physical model incorporating both cell geometry and intraluminal pressure can account for our observations as well as cases in which pressure plays a dominant role.

Suggested Citation

  • Claudia G. Vasquez & Vipul T. Vachharajani & Carlos Garzon-Coral & Alexander R. Dunn, 2021. "Physical basis for the determination of lumen shape in a simple epithelium," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25050-3
    DOI: 10.1038/s41467-021-25050-3
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    Cited by:

    1. Gawoon Shim & Isaac B. Breinyn & Alejandro Martínez-Calvo & Sameeksha Rao & Daniel J. Cohen, 2024. "Bioelectric stimulation controls tissue shape and size," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Ariadna Marín-Llauradó & Sohan Kale & Adam Ouzeri & Tom Golde & Raimon Sunyer & Alejandro Torres-Sánchez & Ernest Latorre & Manuel Gómez-González & Pere Roca-Cusachs & Marino Arroyo & Xavier Trepat, 2023. "Mapping mechanical stress in curved epithelia of designed size and shape," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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