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Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10

Author

Listed:
  • Kirsten C. Boddy

    (Hospital for Sick Children
    University of Toronto)

  • Hongxian Zhu

    (Hospital for Sick Children
    University of Toronto)

  • Vanessa M. D’Costa

    (Hospital for Sick Children
    University of Ottawa
    University of Ottawa)

  • Caishuang Xu

    (University of Saskatchewan)

  • Ksenia Beyrakhova

    (University of Saskatchewan)

  • Miroslaw Cygler

    (University of Saskatchewan)

  • Sergio Grinstein

    (Hospital for Sick Children
    University of Toronto
    University of Toronto)

  • Etienne Coyaud

    (University Health Network)

  • Estelle M. N. Laurent

    (University Health Network)

  • Jonathan St-Germain

    (University Health Network)

  • Brian Raught

    (University Health Network
    University of Toronto)

  • John H. Brumell

    (Hospital for Sick Children
    University of Toronto
    University of Toronto
    Hospital for Sick Children)

Abstract

Salmonella utilizes translocated virulence proteins (termed effectors) to promote host cell invasion. The effector SopD contributes to invasion by promoting scission of the plasma membrane, generating Salmonella-containing vacuoles. SopD is expressed in all Salmonella lineages and plays important roles in animal models of infection, but its host cell targets are unknown. Here we show that SopD can bind to and inhibit the small GTPase Rab10, through a C-terminal GTPase activating protein (GAP) domain. During infection, Rab10 and its effectors MICAL-L1 and EHBP1 are recruited to invasion sites. By inhibiting Rab10, SopD promotes removal of Rab10 and recruitment of Dynamin-2 to drive scission of the plasma membrane. Together, our study uncovers an important role for Rab10 in regulating plasma membrane scission and identifies the mechanism used by a bacterial pathogen to manipulate this function during infection.

Suggested Citation

  • Kirsten C. Boddy & Hongxian Zhu & Vanessa M. D’Costa & Caishuang Xu & Ksenia Beyrakhova & Miroslaw Cygler & Sergio Grinstein & Etienne Coyaud & Estelle M. N. Laurent & Jonathan St-Germain & Brian Raug, 2021. "Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24983-z
    DOI: 10.1038/s41467-021-24983-z
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    Cited by:

    1. Hongxian Zhu & Andrew M. Sydor & Kirsten C. Boddy & Etienne Coyaud & Estelle M. N. Laurent & Aaron Au & Joel M. J. Tan & Bing-Ru Yan & Jason Moffat & Aleixo M. Muise & Christopher M. Yip & Sergio Grin, 2024. "Salmonella exploits membrane reservoirs for invasion of host cells," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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