IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-24978-w.html
   My bibliography  Save this article

The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors

Author

Listed:
  • Xuelei Lai

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Rosario Vega-Léon

    (Humboldt-Universität zu Berlin)

  • Veronique Hugouvieux

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Romain Blanc-Mathieu

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Froukje Wal

    (Wageningen University and Research)

  • Jérémy Lucas

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Catarina S. Silva

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV
    European Molecular Biology Laboratory)

  • Agnès Jourdain

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Jose M. Muino

    (Humboldt-Universität zu Berlin)

  • Max H. Nanao

    (Structural Biology Group)

  • Richard Immink

    (Wageningen University and Research
    Wageningen University and Research)

  • Kerstin Kaufmann

    (Humboldt-Universität zu Berlin)

  • François Parcy

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

  • Cezary Smaczniak

    (Humboldt-Universität zu Berlin)

  • Chloe Zubieta

    (Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV)

Abstract

The MADS transcription factors (TF) are an ancient eukaryotic protein family. In plants, the family is divided into two main lineages. Here, we demonstrate that DNA binding in both lineages absolutely requires a short amino acid sequence C-terminal to the MADS domain (M domain) called the Intervening domain (I domain) that was previously defined only in type II lineage MADS. Structural elucidation of the MI domains from the floral regulator, SEPALLATA3 (SEP3), shows a conserved fold with the I domain acting to stabilise the M domain. Using the floral organ identity MADS TFs, SEP3, APETALA1 (AP1) and AGAMOUS (AG), domain swapping demonstrate that the I domain alters genome-wide DNA-binding specificity and dimerisation specificity. Introducing AG carrying the I domain of AP1 in the Arabidopsis ap1 mutant resulted in strong complementation and restoration of first and second whorl organs. Taken together, these data demonstrate that the I domain acts as an integral part of the DNA-binding domain and significantly contributes to the functional identity of the MADS TF.

Suggested Citation

  • Xuelei Lai & Rosario Vega-Léon & Veronique Hugouvieux & Romain Blanc-Mathieu & Froukje Wal & Jérémy Lucas & Catarina S. Silva & Agnès Jourdain & Jose M. Muino & Max H. Nanao & Richard Immink & Kerstin, 2021. "The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24978-w
    DOI: 10.1038/s41467-021-24978-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-24978-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-24978-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yan Wang & Xiaohua Yao & Youhua Yao & Yixiong Bai & Likun An & Xin Li & Yongmei Cui & Kunlun Wu, 2023. "QTL localisation of seed-related traits in Tibetan hulless barley based on a high-density single-nucleotide polymorphism genetic map," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 59(2), pages 95-108.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24978-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.