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Non-invasive hydrodynamic imaging in plant roots at cellular resolution

Author

Listed:
  • Flavius C. Pascut

    (University of Nottingham)

  • Valentin Couvreur

    (Université catholique de Louvain)

  • Daniela Dietrich

    (University of Bristol)

  • Nicky Leftley

    (University of Nottingham)

  • Guilhem Reyt

    (University of Nottingham
    University of Nottingham)

  • Yann Boursiac

    (BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro)

  • Monica Calvo-Polanco

    (BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro
    University of Salamanca)

  • Ilda Casimiro

    (Universidad de Extremadura, Facultad de Ciencias)

  • Christophe Maurel

    (BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro)

  • David E. Salt

    (University of Nottingham
    University of Nottingham)

  • Xavier Draye

    (Université catholique de Louvain)

  • Darren M. Wells

    (University of Nottingham
    University of Nottingham)

  • Malcolm J. Bennett

    (University of Nottingham
    University of Nottingham)

  • Kevin F. Webb

    (University of Nottingham)

Abstract

A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’.

Suggested Citation

  • Flavius C. Pascut & Valentin Couvreur & Daniela Dietrich & Nicky Leftley & Guilhem Reyt & Yann Boursiac & Monica Calvo-Polanco & Ilda Casimiro & Christophe Maurel & David E. Salt & Xavier Draye & Darr, 2021. "Non-invasive hydrodynamic imaging in plant roots at cellular resolution," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24913-z
    DOI: 10.1038/s41467-021-24913-z
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