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Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks

Author

Listed:
  • Raziye Karapinar

    (Ruhr-University Bochum
    Imperial College London
    The Francis Crick Institute)

  • Jan Claudius Schwitalla

    (Ruhr-University Bochum)

  • Dennis Eickelbeck

    (Ruhr-University Bochum
    Imperial College London
    The Francis Crick Institute)

  • Johanna Pakusch

    (Ruhr-University Bochum)

  • Brix Mücher

    (Ruhr-University Bochum)

  • Michelle Grömmke

    (Ruhr-University Bochum)

  • Tatjana Surdin

    (Ruhr-University Bochum)

  • Thomas Knöpfel

    (Imperial College London)

  • Melanie D. Mark

    (Ruhr-University Bochum)

  • Ida Siveke

    (Ruhr-University Bochum
    University Hospital Essen)

  • Stefan Herlitze

    (Ruhr-University Bochum)

Abstract

Opn7b is a non-visual G protein-coupled receptor expressed in zebrafish. Here we find that Opn7b expressed in HEK cells constitutively activates the Gi/o pathway and illumination with blue/green light inactivates G protein-coupled inwardly rectifying potassium channels. This suggests that light acts as an inverse agonist for Opn7b and can be used as an optogenetic tool to inhibit neuronal networks in the dark and interrupt constitutive inhibition in the light. Consistent with this prediction, illumination of recombinant expressed Opn7b in cortical pyramidal cells results in increased neuronal activity. In awake mice, light stimulation of Opn7b expressed in pyramidal cells of somatosensory cortex reliably induces generalized epileptiform activity within a short (

Suggested Citation

  • Raziye Karapinar & Jan Claudius Schwitalla & Dennis Eickelbeck & Johanna Pakusch & Brix Mücher & Michelle Grömmke & Tatjana Surdin & Thomas Knöpfel & Melanie D. Mark & Ida Siveke & Stefan Herlitze, 2021. "Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24718-0
    DOI: 10.1038/s41467-021-24718-0
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