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Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori

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  • Sandy R. Pernitzsch

    (Institute of Molecular Infection Biology (IMIB), University of Würzburg
    SCIGRAPHIX - Scientific Illustrations)

  • Mona Alzheimer

    (Institute of Molecular Infection Biology (IMIB), University of Würzburg)

  • Belinda U. Bremer

    (Institute of Molecular Infection Biology (IMIB), University of Würzburg)

  • Marie Robbe-Saule

    (Institut Pasteur, Helicobacter Pathogenesis Unit, Microbiology Department
    Equipe Atip-Avenir, Centre Régional de Recherche en Cancérologie Nantes/Angers, INSERM U892, CNRS U6299)

  • Hilde De Reuse

    (Institut Pasteur, Helicobacter Pathogenesis Unit, Microbiology Department)

  • Cynthia M. Sharma

    (Institute of Molecular Infection Biology (IMIB), University of Würzburg)

Abstract

The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase variation by also modulating the expression of a gene (hp0102) that is co-transcribed with tlpB. The hp0102 gene encodes a glycosyltransferase required for LPS O-chain biosynthesis and in vivo colonization of the mouse stomach. The G-repeat length defines a gradual (rather than ON/OFF) control of LPS biosynthesis by RepG, and leads to gradual resistance to a membrane-targeting antibiotic. Thus, RepG-mediated modulation of LPS structure might impact host immune recognition and antibiotic sensitivity, thereby helping H. pylori to adapt and persist in the host.

Suggested Citation

  • Sandy R. Pernitzsch & Mona Alzheimer & Belinda U. Bremer & Marie Robbe-Saule & Hilde De Reuse & Cynthia M. Sharma, 2021. "Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24689-2
    DOI: 10.1038/s41467-021-24689-2
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    Cited by:

    1. Carmen Aguilar & Mindaugas Pauzuolis & Malvika Pompaiah & Ehsan Vafadarnejad & Panagiota Arampatzi & Mara Fischer & Dominik Narres & Mastura Neyazi & Özge Kayisoglu & Thomas Sell & Nils Blüthgen & Mar, 2022. "Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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