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Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders

Author

Listed:
  • Yalda Afshar

    (University of California, Los Angeles)

  • Jiantong Dong

    (University of California, Los Angeles
    Peking University)

  • Pan Zhao

    (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital)

  • Lei Li

    (University of California, Los Angeles
    Shandong Provincial Hospital Affiliated to Shandong University)

  • Shan Wang

    (University of California, Los Angeles
    Shandong Provincial Hospital Affiliated to Shandong University)

  • Ryan Y. Zhang

    (University of California, Los Angeles)

  • Ceng Zhang

    (University of California, Los Angeles)

  • Ophelia Yin

    (University of California, Los Angeles)

  • Christina S. Han

    (University of California, Los Angeles
    Center for Fetal Medicine and Women’s Ultrasound)

  • Brett D. Einerson

    (University of Utah Health)

  • Tania L. Gonzalez

    (Cedars-Sinai Medical Center)

  • Huirong Zhang

    (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital)

  • Anqi Zhou

    (University of California, Los Angeles)

  • Zhuo Yang

    (University of California, Los Angeles)

  • Shih-Jie Chou

    (University of California, Los Angeles)

  • Na Sun

    (University of California, Los Angeles)

  • Ju Cheng

    (University of California, Los Angeles)

  • Henan Zhu

    (University of California, Los Angeles)

  • Jing Wang

    (University of California, Los Angeles)

  • Tiffany X. Zhang

    (University of California, Los Angeles)

  • Yi-Te Lee

    (University of California, Los Angeles)

  • Jasmine J. Wang

    (Cedars-Sinai Medical Center)

  • Pai-Chi Teng

    (Cedars-Sinai Medical Center)

  • Peng Yang

    (University of California, Los Angeles)

  • Dongping Qi

    (University of California, Los Angeles)

  • Meiping Zhao

    (Peking University)

  • Myung-Shin Sim

    (University of California, Los Angeles)

  • Ruilian Zhe

    (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital)

  • Jeffrey D. Goldstein

    (University of California, Los Angeles)

  • John Williams

    (Cedars-Sinai Medical Center)

  • Xietong Wang

    (Shandong Provincial Hospital Affiliated to Shandong University)

  • Qingying Zhang

    (University of California, Los Angeles
    Fudan University)

  • Lawrence D. Platt

    (University of California, Los Angeles
    Center for Fetal Medicine and Women’s Ultrasound)

  • Chang Zou

    (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital)

  • Margareta D. Pisarska

    (Cedars-Sinai Medical Center)

  • Hsian-Rong Tseng

    (University of California, Los Angeles)

  • Yazhen Zhu

    (University of California, Los Angeles)

Abstract

Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report a nanostructure-embedded microchip that efficiently enriches both single and clustered circulating trophoblasts (cTBs) from maternal blood for detecting PAS. We discover a uniquely high prevalence of cTB-clusters in PAS and subsequently optimize the device to preserve the intactness of these clusters. Our feasibility study on the enumeration of cTBs and cTB-clusters from 168 pregnant women demonstrates excellent diagnostic performance for distinguishing PAS from non-PAS. A logistic regression model is constructed using a training cohort and then cross-validated and tested using an independent cohort. The combined cTB assay achieves an Area Under ROC Curve of 0.942 (throughout gestation) and 0.924 (early gestation) for distinguishing PAS from non-PAS. Our assay holds the potential to improve current diagnostic modalities for the early detection of PAS.

Suggested Citation

  • Yalda Afshar & Jiantong Dong & Pan Zhao & Lei Li & Shan Wang & Ryan Y. Zhang & Ceng Zhang & Ophelia Yin & Christina S. Han & Brett D. Einerson & Tania L. Gonzalez & Huirong Zhang & Anqi Zhou & Zhuo Ya, 2021. "Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24627-2
    DOI: 10.1038/s41467-021-24627-2
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