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Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2

Author

Listed:
  • Masahiro Naganuma

    (The University of Tokyo
    RIKEN Center for Biosystems Dynamics Research)

  • Hisashi Tadakuma

    (The University of Tokyo
    ShanghaiTech University)

  • Yukihide Tomari

    (The University of Tokyo
    The University of Tokyo)

Abstract

Drosophila Dicer-2 (Dcr-2) produces small interfering RNAs from long double-stranded RNAs (dsRNAs), playing an essential role in antiviral RNA interference. The dicing reaction by Dcr-2 is enhanced by Loquacious-PD (Loqs-PD), a dsRNA-binding protein that partners with Dcr-2. Previous biochemical analyses have proposed that Dcr-2 uses two distinct—processive or distributive—modes of cleavage by distinguishing the terminal structures of dsRNAs and that Loqs-PD alters the terminal dependence of Dcr-2. However, the direct evidence for this model is lacking, as the dynamic movement of Dcr-2 along dsRNAs has not been traced. Here, by utilizing single-molecule imaging, we show that the terminal structures of long dsRNAs and the presence or absence of Loqs-PD do not essentially change Dcr-2’s cleavage mode between processive and distributive, but rather simply affect the probability for Dcr-2 to undergo the cleavage reaction. Our results provide a refined model for how the dicing reaction by Dcr-2 is regulated.

Suggested Citation

  • Masahiro Naganuma & Hisashi Tadakuma & Yukihide Tomari, 2021. "Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24555-1
    DOI: 10.1038/s41467-021-24555-1
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    Cited by:

    1. Ting Deng & Shichen Su & Xun Yuan & Jinqiu He & Ying Huang & Jinbiao Ma & Jia Wang, 2023. "Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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