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M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity

Author

Listed:
  • Indira Bag

    (Nuclear Organization and Gene Expression Section
    Laboratory of Biochemistry and Genetics)

  • Shue Chen

    (Nuclear Organization and Gene Expression Section
    Laboratory of Biochemistry and Genetics)

  • Leah F. Rosin

    (Nuclear Organization and Gene Expression Section
    Laboratory of Biochemistry and Genetics)

  • Yang Chen

    (Nuclear Organization and Gene Expression Section
    Laboratory of Biochemistry and Genetics)

  • Chen-Yu Liu

    (Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health)

  • Guo-Yun Yu

    (Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health)

  • Elissa P. Lei

    (Nuclear Organization and Gene Expression Section
    Laboratory of Biochemistry and Genetics)

Abstract

Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.

Suggested Citation

  • Indira Bag & Shue Chen & Leah F. Rosin & Yang Chen & Chen-Yu Liu & Guo-Yun Yu & Elissa P. Lei, 2021. "M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24407-y
    DOI: 10.1038/s41467-021-24407-y
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    Cited by:

    1. Gabriela Poliacikova & Marine Barthez & Thomas Rival & Aïcha Aouane & Nuno Miguel Luis & Fabrice Richard & Fabrice Daian & Nicolas Brouilly & Frank Schnorrer & Corinne Maurel-Zaffran & Yacine Graba & , 2023. "M1BP is an essential transcriptional activator of oxidative metabolism during Drosophila development," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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