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Probing the biogenesis pathway and dynamics of thylakoid membranes

Author

Listed:
  • Tuomas Huokko

    (University of Liverpool)

  • Tao Ni

    (University of Oxford)

  • Gregory F. Dykes

    (University of Liverpool)

  • Deborah M. Simpson

    (University of Liverpool)

  • Philip Brownridge

    (University of Liverpool)

  • Fabian D. Conradi

    (Queen Mary University of London)

  • Robert J. Beynon

    (University of Liverpool)

  • Peter J. Nixon

    (Imperial College London)

  • Conrad W. Mullineaux

    (Queen Mary University of London)

  • Peijun Zhang

    (University of Oxford
    Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus)

  • Lu-Ning Liu

    (University of Liverpool
    Ocean University of China)

Abstract

How thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, and probe the spatial-temporal stepwise biogenesis process of thylakoid membranes in cells. Our results reveal that the plasma membrane and regularly arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments emerge between the plasma membrane and pre-existing thylakoids. Photosystem I monomers appear in the thylakoid membranes earlier than other mature photosystem assemblies, followed by generation of Photosystem I trimers and Photosystem II complexes. Redistribution of photosynthetic complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid network. This study provides insights into the dynamic biogenesis process and maturation of the functional photosynthetic machinery.

Suggested Citation

  • Tuomas Huokko & Tao Ni & Gregory F. Dykes & Deborah M. Simpson & Philip Brownridge & Fabian D. Conradi & Robert J. Beynon & Peter J. Nixon & Conrad W. Mullineaux & Peijun Zhang & Lu-Ning Liu, 2021. "Probing the biogenesis pathway and dynamics of thylakoid membranes," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23680-1
    DOI: 10.1038/s41467-021-23680-1
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    Cited by:

    1. Yu-Zhong Zhang & Kang Li & Bing-Yue Qin & Jian-Ping Guo & Quan-Bao Zhang & Dian-Li Zhao & Xiu-Lan Chen & Jun Gao & Lu-Ning Liu & Long-Sheng Zhao, 2024. "Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Lucas Gewehr & Benedikt Junglas & Ruven Jilly & Johannes Franz & Wenyu Eva Zhu & Tobias Weidner & Mischa Bonn & Carsten Sachse & Dirk Schneider, 2023. "SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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