IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-23680-1.html

Some searches may not work properly. We apologize for the inconvenience.

   My bibliography  Save this article

Probing the biogenesis pathway and dynamics of thylakoid membranes

Author

Listed:
  • Tuomas Huokko

    (University of Liverpool)

  • Tao Ni

    (University of Oxford)

  • Gregory F. Dykes

    (University of Liverpool)

  • Deborah M. Simpson

    (University of Liverpool)

  • Philip Brownridge

    (University of Liverpool)

  • Fabian D. Conradi

    (Queen Mary University of London)

  • Robert J. Beynon

    (University of Liverpool)

  • Peter J. Nixon

    (Imperial College London)

  • Conrad W. Mullineaux

    (Queen Mary University of London)

  • Peijun Zhang

    (University of Oxford
    Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus)

  • Lu-Ning Liu

    (University of Liverpool
    Ocean University of China)

Abstract

How thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, and probe the spatial-temporal stepwise biogenesis process of thylakoid membranes in cells. Our results reveal that the plasma membrane and regularly arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments emerge between the plasma membrane and pre-existing thylakoids. Photosystem I monomers appear in the thylakoid membranes earlier than other mature photosystem assemblies, followed by generation of Photosystem I trimers and Photosystem II complexes. Redistribution of photosynthetic complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid network. This study provides insights into the dynamic biogenesis process and maturation of the functional photosynthetic machinery.

Suggested Citation

  • Tuomas Huokko & Tao Ni & Gregory F. Dykes & Deborah M. Simpson & Philip Brownridge & Fabian D. Conradi & Robert J. Beynon & Peter J. Nixon & Conrad W. Mullineaux & Peijun Zhang & Lu-Ning Liu, 2021. "Probing the biogenesis pathway and dynamics of thylakoid membranes," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23680-1
    DOI: 10.1038/s41467-021-23680-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-23680-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-23680-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yu-Zhong Zhang & Kang Li & Bing-Yue Qin & Jian-Ping Guo & Quan-Bao Zhang & Dian-Li Zhao & Xiu-Lan Chen & Jun Gao & Lu-Ning Liu & Long-Sheng Zhao, 2024. "Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Lucas Gewehr & Benedikt Junglas & Ruven Jilly & Johannes Franz & Wenyu Eva Zhu & Tobias Weidner & Mischa Bonn & Carsten Sachse & Dirk Schneider, 2023. "SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23680-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.