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RETRACTED ARTICLE: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells

Author

Listed:
  • Ruifeng Zhao

    (Yangzhou University
    Yangzhou University)

  • Qisheng Zuo

    (Yangzhou University
    Yangzhou University)

  • Xia Yuan

    (Yangzhou University
    Yangzhou University)

  • Kai Jin

    (Yangzhou University
    Yangzhou University)

  • Jing Jin

    (Yangzhou University
    Yangzhou University)

  • Ying Ding

    (Yangzhou University
    Yangzhou University)

  • Chen Zhang

    (Yangzhou University
    Yangzhou University)

  • Tingting Li

    (Yangzhou University
    Yangzhou University)

  • Jingyi Jiang

    (Yangzhou University
    Yangzhou University)

  • Jiancheng Li

    (Yangzhou University
    Yangzhou University)

  • Ming Zhang

    (Yangzhou University
    Yangzhou University)

  • Xiang Shi

    (Yangzhou University
    Yangzhou University)

  • Hongyan Sun

    (Yangzhou University
    Yangzhou University)

  • Yani Zhang

    (Yangzhou University
    Yangzhou University)

  • Qi Xu

    (Yangzhou University
    Yangzhou University)

  • Guobin Chang

    (Yangzhou University
    Yangzhou University)

  • Zhenhua Zhao

    (The Poultry Research Institute of Chinese Academy of Agricultural Sciences)

  • Bing Li

    (The Poultry Research Institute of Chinese Academy of Agricultural Sciences)

  • Xinsheng Wu

    (Yangzhou University
    Yangzhou University)

  • Yang Zhang

    (Yangzhou University
    Yangzhou University)

  • Jiuzhou Song

    (University of Maryland)

  • Guohong Chen

    (Yangzhou University
    Yangzhou University)

  • Bichun Li

    (Yangzhou University
    Yangzhou University)

Abstract

The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor’s characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.

Suggested Citation

  • Ruifeng Zhao & Qisheng Zuo & Xia Yuan & Kai Jin & Jing Jin & Ying Ding & Chen Zhang & Tingting Li & Jingyi Jiang & Jiancheng Li & Ming Zhang & Xiang Shi & Hongyan Sun & Yani Zhang & Qi Xu & Guobin Cha, 2021. "RETRACTED ARTICLE: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23242-5
    DOI: 10.1038/s41467-021-23242-5
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