IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-23002-5.html
   My bibliography  Save this article

A sand fly salivary protein acts as a neutrophil chemoattractant

Author

Listed:
  • Anderson B. Guimaraes-Costa

    (National Institutes of Health
    Universidade Federal do Rio de Janeiro)

  • John P. Shannon

    (National Institutes of Health)

  • Ingrid Waclawiak

    (Universidade Federal do Rio de Janeiro)

  • Jullyanna Oliveira

    (Universidade Federal do Rio de Janeiro)

  • Claudio Meneses

    (National Institutes of Health)

  • Waldione Castro

    (National Institutes of Health)

  • Xi Wen

    (National Institute of Allergy and Infectious Diseases, NIH)

  • Joseph Brzostowski

    (National Institute of Allergy and Infectious Diseases, NIH)

  • Tiago D. Serafim

    (National Institutes of Health)

  • John F. Andersen

    (National Institutes of Health)

  • Heather D. Hickman

    (National Institutes of Health)

  • Shaden Kamhawi

    (National Institutes of Health)

  • Jesus G. Valenzuela

    (National Institutes of Health)

  • Fabiano Oliveira

    (National Institutes of Health)

Abstract

Apart from bacterial formyl peptides or viral chemokine mimicry, a non-vertebrate or insect protein that directly attracts mammalian innate cells such as neutrophils has not been molecularly characterized. Here, we show that members of sand fly yellow salivary proteins induce in vitro chemotaxis of mouse, canine and human neutrophils in transwell migration or EZ-TAXIScan assays. We demonstrate murine neutrophil recruitment in vivo using flow cytometry and two-photon intravital microscopy in Lysozyme-M-eGFP transgenic mice. We establish that the structure of this ~ 45 kDa neutrophil chemotactic protein does not resemble that of known chemokines. This chemoattractant acts through a G-protein-coupled receptor and is dependent on calcium influx. Of significance, this chemoattractant protein enhances lesion pathology (P

Suggested Citation

  • Anderson B. Guimaraes-Costa & John P. Shannon & Ingrid Waclawiak & Jullyanna Oliveira & Claudio Meneses & Waldione Castro & Xi Wen & Joseph Brzostowski & Tiago D. Serafim & John F. Andersen & Heather , 2021. "A sand fly salivary protein acts as a neutrophil chemoattractant," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23002-5
    DOI: 10.1038/s41467-021-23002-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-23002-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-23002-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23002-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.