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Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis

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Listed:
  • Nicolas Butel

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay
    Université Paris-Sud, Université Paris-Saclay)

  • Agnès Yu

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay)

  • Ivan Le Masson

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay)

  • Filipe Borges

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay)

  • Taline Elmayan

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay)

  • Christelle Taochy

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay
    The University of Queensland)

  • Nial R. Gursanscky

    (The University of Queensland)

  • Jiangling Cao

    (The University of Queensland)

  • Shengnan Bi

    (The University of Queensland)

  • Anne Sawyer

    (The University of Queensland
    The University of Queensland)

  • Bernard J. Carroll

    (The University of Queensland)

  • Hervé Vaucheret

    (UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay)

Abstract

Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes.

Suggested Citation

  • Nicolas Butel & Agnès Yu & Ivan Le Masson & Filipe Borges & Taline Elmayan & Christelle Taochy & Nial R. Gursanscky & Jiangling Cao & Shengnan Bi & Anne Sawyer & Bernard J. Carroll & Hervé Vaucheret, 2021. "Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22995-3
    DOI: 10.1038/s41467-021-22995-3
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