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Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals

Author

Listed:
  • Yan Li

    (Chinese Academy of Sciences (CAS))

  • Aymeric Leveau

    (John Innes Centre, Norwich Research Park)

  • Qiang Zhao

    (Chinese Academy of Sciences (CAS))

  • Qi Feng

    (Chinese Academy of Sciences (CAS))

  • Hengyun Lu

    (Chinese Academy of Sciences (CAS))

  • Jiashun Miao

    (Chinese Academy of Sciences (CAS))

  • Zheyong Xue

    (John Innes Centre, Norwich Research Park)

  • Azahara C. Martin

    (John Innes Centre, Norwich Research Park)

  • Eva Wegel

    (John Innes Centre, Norwich Research Park)

  • Jing Wang

    (John Innes Centre, Norwich Research Park)

  • Anastasia Orme

    (John Innes Centre, Norwich Research Park)

  • Maria-Dolores Rey

    (John Innes Centre, Norwich Research Park)

  • Miroslava Karafiátová

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Jan Vrána

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Burkhard Steuernagel

    (John Innes Centre, Norwich Research Park)

  • Ryan Joynson

    (Earlham Institute, Norwich Research Park)

  • Charlotte Owen

    (John Innes Centre, Norwich Research Park)

  • James Reed

    (John Innes Centre, Norwich Research Park)

  • Thomas Louveau

    (John Innes Centre, Norwich Research Park)

  • Michael J. Stephenson

    (John Innes Centre, Norwich Research Park)

  • Lei Zhang

    (Chinese Academy of Sciences (CAS))

  • Xuehui Huang

    (Chinese Academy of Sciences (CAS))

  • Tao Huang

    (Chinese Academy of Sciences (CAS))

  • Danling Fan

    (Chinese Academy of Sciences (CAS))

  • Congcong Zhou

    (Chinese Academy of Sciences (CAS))

  • Qilin Tian

    (Chinese Academy of Sciences (CAS))

  • Wenjun Li

    (Chinese Academy of Sciences (CAS))

  • Yiqi Lu

    (Chinese Academy of Sciences (CAS))

  • Jiaying Chen

    (Chinese Academy of Sciences (CAS))

  • Yan Zhao

    (Chinese Academy of Sciences (CAS))

  • Ying Lu

    (Chinese Academy of Sciences (CAS))

  • Chuanrang Zhu

    (Chinese Academy of Sciences (CAS))

  • Zhenhua Liu

    (Shanghai Jiao Tong University)

  • Guy Polturak

    (John Innes Centre, Norwich Research Park)

  • Rebecca Casson

    (John Innes Centre, Norwich Research Park)

  • Lionel Hill

    (John Innes Centre, Norwich Research Park)

  • Graham Moore

    (John Innes Centre, Norwich Research Park)

  • Rachel Melton

    (John Innes Centre, Norwich Research Park)

  • Neil Hall

    (Earlham Institute, Norwich Research Park)

  • Brande B. H. Wulff

    (John Innes Centre, Norwich Research Park)

  • Jaroslav Doležel

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Tim Langdon

    (Aberystwyth University, Gogerddan)

  • Bin Han

    (Chinese Academy of Sciences (CAS))

  • Anne Osbourn

    (John Innes Centre, Norwich Research Park)

Abstract

Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat—the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a ‘self-poisoning’ scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity.

Suggested Citation

  • Yan Li & Aymeric Leveau & Qiang Zhao & Qi Feng & Hengyun Lu & Jiashun Miao & Zheyong Xue & Azahara C. Martin & Eva Wegel & Jing Wang & Anastasia Orme & Maria-Dolores Rey & Miroslava Karafiátová & Jan , 2021. "Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22920-8
    DOI: 10.1038/s41467-021-22920-8
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    Cited by:

    1. Chun Zhang & Nicholas A. Johnson & Nathan Hall & Xingshan Tian & Qin Yu & Eric L. Patterson, 2023. "Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Abigail E. Bryson & Emily R. Lanier & Kin H. Lau & John P. Hamilton & Brieanne Vaillancourt & Davis Mathieu & Alan E. Yocca & Garret P. Miller & Patrick P. Edger & C. Robin Buell & Björn Hamberger, 2023. "Uncovering a miltiradiene biosynthetic gene cluster in the Lamiaceae reveals a dynamic evolutionary trajectory," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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