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Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli

Author

Listed:
  • Vijay Saxena

    (Indiana University School of Medicine, Department of Pediatrics, Division of Nephrology)

  • Hongyu Gao

    (Indiana University School of Medicine, Department of Medical & Molecular Genetics)

  • Samuel Arregui

    (Indiana University School of Medicine, Department of Pediatrics, Division of Nephrology)

  • Amy Zollman

    (Indiana University School of Medicine, Department of Medicine, Division of Nephrology)

  • Malgorzata Maria Kamocka

    (Indiana University School of Medicine, Department of Medicine, Division of Nephrology)

  • Xiaoling Xuei

    (Indiana University School of Medicine, Department of Medical & Molecular Genetics)

  • Patrick McGuire

    (Indiana University School of Medicine, Department of Medical & Molecular Genetics)

  • Michael Hutchens

    (Oregon Health and Science University, Department of Anesthesiology & Perioperative Medicine)

  • Takashi Hato

    (Indiana University School of Medicine, Department of Medicine, Division of Nephrology)

  • David S. Hains

    (Indiana University School of Medicine, Department of Pediatrics, Division of Nephrology)

  • Andrew L. Schwaderer

    (Indiana University School of Medicine, Department of Pediatrics, Division of Nephrology)

Abstract

Kidney intercalated cells are involved in acid-base homeostasis via vacuolar ATPase expression. Here we report six human intercalated cell subtypes, including hybrid principal-intercalated cells identified from single cell transcriptomics. Phagosome maturation is a biological process that increases in biological pathway analysis rank following exposure to uropathogenic Escherichia coli in two of the intercalated cell subtypes. Real time confocal microscopy visualization of murine renal tubules perfused with green fluorescent protein expressing Escherichia coli or pHrodo Green E. coli BioParticles demonstrates that intercalated cells actively phagocytose bacteria then acidify phagolysosomes. Additionally, intercalated cells have increased vacuolar ATPase expression following in vivo experimental UTI. Taken together, intercalated cells exhibit a transcriptional response conducive to the kidney’s defense, engulf bacteria and acidify the internalized bacteria. Intercalated cells represent an epithelial cell with characteristics of professional phagocytes like macrophages.

Suggested Citation

  • Vijay Saxena & Hongyu Gao & Samuel Arregui & Amy Zollman & Malgorzata Maria Kamocka & Xiaoling Xuei & Patrick McGuire & Michael Hutchens & Takashi Hato & David S. Hains & Andrew L. Schwaderer, 2021. "Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22672-5
    DOI: 10.1038/s41467-021-22672-5
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    Cited by:

    1. Victor Hugo Canela & William S. Bowen & Ricardo Melo Ferreira & Farooq Syed & James E. Lingeman & Angela R. Sabo & Daria Barwinska & Seth Winfree & Blue B. Lake & Ying-Hua Cheng & Joseph P. Gaut & Mic, 2023. "A spatially anchored transcriptomic atlas of the human kidney papilla identifies significant immune injury in patients with stone disease," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Daniel Charytonowicz & Rachel Brody & Robert Sebra, 2023. "Interpretable and context-free deconvolution of multi-scale whole transcriptomic data with UniCell deconvolve," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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