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Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

Author

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  • Kazuki Motomura

    (Ritsumeikan Global Innovation Research Organization, Ritsumeikan University
    Nagoya University, Furo-cho, Chikusa-ku
    JST, PRESTO)

  • Hidenori Takeuchi

    (Nagoya University, Furo-cho, Chikusa-ku
    Nagoya University, Furo-cho, Chikusa-ku)

  • Michitaka Notaguchi

    (Nagoya University, Furo-cho, Chikusa-ku
    Nagoya University, Furo-cho, Chikusa-ku)

  • Haruna Tsuchi

    (Yokohama City University, Maioka-cho, Totsuka-ku)

  • Atsushi Takeda

    (Ritsumeikan Global Innovation Research Organization, Ritsumeikan University
    Ritsumeikan University)

  • Tetsu Kinoshita

    (Yokohama City University, Maioka-cho, Totsuka-ku)

  • Tetsuya Higashiyama

    (Nagoya University, Furo-cho, Chikusa-ku
    Nagoya University, Furo-cho, Chikusa-ku
    The University of Tokyo, Hongo, Bunkyo-ku)

  • Daisuke Maruyama

    (Yokohama City University, Maioka-cho, Totsuka-ku)

Abstract

During the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

Suggested Citation

  • Kazuki Motomura & Hidenori Takeuchi & Michitaka Notaguchi & Haruna Tsuchi & Atsushi Takeda & Tetsu Kinoshita & Tetsuya Higashiyama & Daisuke Maruyama, 2021. "Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22661-8
    DOI: 10.1038/s41467-021-22661-8
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    Cited by:

    1. Xiangfei Wang & Tonghui Li & Jiuting Xu & Fanfan Zhang & Lifang Liu & Ting Wang & Chun Wang & Haiyun Ren & Yi Zhang, 2024. "Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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