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Selectivity of mRNA degradation by autophagy in yeast

Author

Listed:
  • Shiho Makino

    (Cell Biology Center, Tokyo Institute of Technology)

  • Tomoko Kawamata

    (Cell Biology Center, Tokyo Institute of Technology)

  • Shintaro Iwasaki

    (RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research
    The University of Tokyo
    AMED-CREST, Japan Agency for Medical Research and Development)

  • Yoshinori Ohsumi

    (Cell Biology Center, Tokyo Institute of Technology)

Abstract

Synthesis and degradation of cellular constituents must be balanced to maintain cellular homeostasis, especially during adaptation to environmental stress. The role of autophagy in the degradation of proteins and organelles is well-characterized. However, autophagy-mediated RNA degradation in response to stress and the potential preference of specific RNAs to undergo autophagy-mediated degradation have not been examined. In this study, we demonstrate selective mRNA degradation by rapamycin-induced autophagy in yeast. Profiling of mRNAs from the vacuole reveals that subsets of mRNAs, such as those encoding amino acid biosynthesis and ribosomal proteins, are preferentially delivered to the vacuole by autophagy for degradation. We also reveal that autophagy-mediated mRNA degradation is tightly coupled with translation by ribosomes. Genome-wide ribosome profiling suggested a high correspondence between ribosome association and targeting to the vacuole. We propose that autophagy-mediated mRNA degradation is a unique and previously-unappreciated function of autophagy that affords post-transcriptional gene regulation.

Suggested Citation

  • Shiho Makino & Tomoko Kawamata & Shintaro Iwasaki & Yoshinori Ohsumi, 2021. "Selectivity of mRNA degradation by autophagy in yeast," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22574-6
    DOI: 10.1038/s41467-021-22574-6
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    Cited by:

    1. Kotaro Tomuro & Mari Mito & Hirotaka Toh & Naohiro Kawamoto & Takahito Miyake & Siu Yu A. Chow & Masao Doi & Yoshiho Ikeuchi & Yuichi Shichino & Shintaro Iwasaki, 2024. "Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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