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Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

Author

Listed:
  • Liliana Barbieri

    (University of Oxford)

  • Huw Colin-York

    (University of Oxford
    University of Oxford)

  • Kseniya Korobchevskaya

    (University of Oxford)

  • Di Li

    (Chinese Academy of Sciences)

  • Deanna L. Wolfson

    (UiT The Arctic University of Norway)

  • Narain Karedla

    (University of Oxford
    Rosalind Franklin Institute)

  • Falk Schneider

    (University of Oxford
    University of Oxford)

  • Balpreet S. Ahluwalia

    (UiT The Arctic University of Norway)

  • Tore Seternes

    (UiT The Arctic University of Norway)

  • Roy A. Dalmo

    (UiT The Arctic University of Norway)

  • Michael L. Dustin

    (University of Oxford)

  • Dong Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Marco Fritzsche

    (University of Oxford
    University of Oxford
    Rosalind Franklin Institute)

Abstract

Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

Suggested Citation

  • Liliana Barbieri & Huw Colin-York & Kseniya Korobchevskaya & Di Li & Deanna L. Wolfson & Narain Karedla & Falk Schneider & Balpreet S. Ahluwalia & Tore Seternes & Roy A. Dalmo & Michael L. Dustin & Do, 2021. "Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22377-9
    DOI: 10.1038/s41467-021-22377-9
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    Cited by:

    1. Pablo F. Céspedes & Ashwin Jainarayanan & Lola Fernández-Messina & Salvatore Valvo & David G. Saliba & Elke Kurz & Audun Kvalvaag & Lina Chen & Charity Ganskow & Huw Colin-York & Marco Fritzsche & Yan, 2022. "T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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