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Astigmatic traction force microscopy (aTFM)

Author

Listed:
  • Di Li

    (Chinese Academy of Sciences)

  • Huw Colin-York

    (University of Oxford
    University of Oxford)

  • Liliana Barbieri

    (University of Oxford)

  • Yousef Javanmardi

    (University College London)

  • Yuting Guo

    (Chinese Academy of Sciences)

  • Kseniya Korobchevskaya

    (University of Oxford)

  • Emad Moeendarbary

    (University College London)

  • Dong Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Marco Fritzsche

    (University of Oxford
    University of Oxford
    Harwell Campus)

Abstract

Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.

Suggested Citation

  • Di Li & Huw Colin-York & Liliana Barbieri & Yousef Javanmardi & Yuting Guo & Kseniya Korobchevskaya & Emad Moeendarbary & Dong Li & Marco Fritzsche, 2021. "Astigmatic traction force microscopy (aTFM)," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22376-w
    DOI: 10.1038/s41467-021-22376-w
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    Cited by:

    1. Johannes W Blumberg & Ulrich S Schwarz, 2022. "Comparison of direct and inverse methods for 2.5D traction force microscopy," PLOS ONE, Public Library of Science, vol. 17(1), pages 1-25, January.
    2. Pablo F. Céspedes & Ashwin Jainarayanan & Lola Fernández-Messina & Salvatore Valvo & David G. Saliba & Elke Kurz & Audun Kvalvaag & Lina Chen & Charity Ganskow & Huw Colin-York & Marco Fritzsche & Yan, 2022. "T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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