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Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants

Author

Listed:
  • Yutaro Shimizu

    (RIKEN Center for Advanced Photonics, Wako
    The University of Tokyo)

  • Junpei Takagi

    (Konan University, Kobe)

  • Emi Ito

    (Ochanomizu University)

  • Yoko Ito

    (RIKEN Center for Advanced Photonics, Wako
    UMR 5200, CNRS, Université de Bordeaux)

  • Kazuo Ebine

    (National Institute for Basic Biology
    SOKENDAI (The Graduate University for Advanced Studies))

  • Yamato Komatsu

    (The University of Tokyo)

  • Yumi Goto

    (Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science)

  • Mayuko Sato

    (Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science)

  • Kiminori Toyooka

    (Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science)

  • Takashi Ueda

    (National Institute for Basic Biology
    SOKENDAI (The Graduate University for Advanced Studies))

  • Kazuo Kurokawa

    (RIKEN Center for Advanced Photonics, Wako)

  • Tomohiro Uemura

    (Ochanomizu University)

  • Akihiko Nakano

    (RIKEN Center for Advanced Photonics, Wako)

Abstract

The trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different destinies still remains elusive. Here, we examined 3D localization and 4D dynamics of TGN-localized proteins of Arabidopsis thaliana that are involved in either secretory or vacuolar trafficking from the TGN, by a multicolor high-speed and high-resolution spinning-disk confocal microscopy approach that we developed. We demonstrate that TGN-localized proteins exhibit spatially and temporally distinct distribution. VAMP721 (R-SNARE), AP (adaptor protein complex)−1, and clathrin which are involved in secretory trafficking compose an exclusive subregion, whereas VAMP727 (R-SNARE) and AP-4 involved in vacuolar trafficking compose another subregion on the same TGN. Based on these findings, we propose that the single TGN has at least two subregions, or “zones”, responsible for distinct cargo sorting: the secretory-trafficking zone and the vacuolar-trafficking zone.

Suggested Citation

  • Yutaro Shimizu & Junpei Takagi & Emi Ito & Yoko Ito & Kazuo Ebine & Yamato Komatsu & Yumi Goto & Mayuko Sato & Kiminori Toyooka & Takashi Ueda & Kazuo Kurokawa & Tomohiro Uemura & Akihiko Nakano, 2021. "Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22267-0
    DOI: 10.1038/s41467-021-22267-0
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