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The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity

Author

Listed:
  • Benoît Bragantini

    (Université de Lorraine, CNRS, IMoPA
    Mayo Clinic)

  • Christophe Charron

    (Université de Lorraine, CNRS, IMoPA)

  • Maxime Bourguet

    (Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS IPHC UMR 7178)

  • Arnaud Paul

    (Université de Lorraine, CNRS, IMoPA)

  • Decebal Tiotiu

    (Université de Lorraine, CNRS, IMoPA)

  • Benjamin Rothé

    (Université de Lorraine, CNRS, IMoPA
    Ecole polytechnique fédérale de Lausanne (EPFL) SV ISREC, Station 19)

  • Hélène Marty

    (Université de Lorraine, CNRS, IMoPA)

  • Guillaume Terral

    (Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS IPHC UMR 7178)

  • Steve Hessmann

    (Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS IPHC UMR 7178)

  • Laurence Decourty

    (Génétique des Interactions Macromoléculaires, Département de Génomes et Génétique, Institut Pasteur (UMR3525-CNRS))

  • Marie-Eve Chagot

    (Université de Lorraine, CNRS, IMoPA)

  • Jean-Marc Strub

    (Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS IPHC UMR 7178)

  • Séverine Massenet

    (Université de Lorraine, CNRS, IMoPA)

  • Edouard Bertrand

    (IGH, CNRS, Université de Montpellier)

  • Marc Quinternet

    (Université de Lorraine, CNRS, INSERM, IBSLor)

  • Cosmin Saveanu

    (Génétique des Interactions Macromoléculaires, Département de Génomes et Génétique, Institut Pasteur (UMR3525-CNRS))

  • Sarah Cianférani

    (Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS IPHC UMR 7178)

  • Stéphane Labialle

    (Université de Lorraine, CNRS, IMoPA)

  • Xavier Manival

    (Université de Lorraine, CNRS, IMoPA)

  • Bruno Charpentier

    (Université de Lorraine, CNRS, IMoPA)

Abstract

Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins initiates co-transcriptionally and requires the action of the assembly machinery including the Hsp90/R2TP complex, the Rsa1p:Hit1p heterodimer and the Bcd1 protein. We present genetic interactions between the Rsa1p-encoding gene and genes involved in chromatin organization including RTT106 that codes for the H3-H4 histone chaperone Rtt106p controlling H3K56ac deposition. We show that Bcd1p binds Rtt106p and controls its transcription-dependent recruitment by reducing its association with RNA polymerase II, modulating H3K56ac levels at gene body. We reveal the 3D structures of the free and Rtt106p-bound forms of Bcd1p using nuclear magnetic resonance and X-ray crystallography. The interaction is also studied by a combination of biophysical and proteomic techniques. Bcd1p interacts with a region that is distinct from the interaction interface between the histone chaperone and histone H3. Our results are evidence for a protein interaction interface for Rtt106p that controls its transcription-associated activity.

Suggested Citation

  • Benoît Bragantini & Christophe Charron & Maxime Bourguet & Arnaud Paul & Decebal Tiotiu & Benjamin Rothé & Hélène Marty & Guillaume Terral & Steve Hessmann & Laurence Decourty & Marie-Eve Chagot & Jea, 2021. "The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22077-4
    DOI: 10.1038/s41467-021-22077-4
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