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A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Author

Listed:
  • Alain Townsend

    (John Radcliffe Hospital
    University of Oxford)

  • Pramila Rijal

    (John Radcliffe Hospital
    University of Oxford)

  • Julie Xiao

    (John Radcliffe Hospital)

  • Tiong Kit Tan

    (John Radcliffe Hospital)

  • Kuan-Ying A. Huang

    (Chang Gung University
    Chang Gung Memorial Hospital)

  • Lisa Schimanski

    (John Radcliffe Hospital
    University of Oxford)

  • Jiandong Huo

    (The Rosalind Franklin Institute)

  • Nimesh Gupta

    (National Institute of Immunology)

  • Rolle Rahikainen

    (University of Oxford)

  • Philippa C. Matthews

    (John Radcliffe Hospital
    University of Oxford)

  • Derrick Crook

    (John Radcliffe Hospital
    University of Oxford, John Radcliffe Hospital
    John Radcliffe Hospital)

  • Sarah Hoosdally

    (University of Oxford
    John Radcliffe Hospital)

  • Susanna Dunachie

    (University of Oxford
    Oxford University Hospitals NHS Foundation Trust)

  • Eleanor Barnes

    (University of Oxford, John Radcliffe Hospital)

  • Teresa Street

    (University of Oxford, John Radcliffe Hospital
    John Radcliffe Hospital)

  • Christopher P. Conlon

    (University of Oxford, John Radcliffe Hospital)

  • John Frater

    (University of Oxford, John Radcliffe Hospital)

  • Carolina V. Arancibia-Cárcamo

    (University of Oxford, John Radcliffe Hospital)

  • Justine Rudkin

    (University of Oxford)

  • Nicole Stoesser

    (John Radcliffe Hospital
    University of Oxford, John Radcliffe Hospital)

  • Fredrik Karpe

    (John Radcliffe Hospital
    University of Oxford)

  • Matthew Neville

    (University of Oxford)

  • Rutger Ploeg

    (University of Oxford, John Radcliffe Hospital)

  • Marta Oliveira

    (University of Oxford, John Radcliffe Hospital)

  • David J. Roberts

    (John Radcliffe Hospital
    University of Oxford, John Radcliffe Hospital)

  • Abigail A. Lamikanra

    (John Radcliffe Hospital)

  • Hoi Pat Tsang

    (John Radcliffe Hospital)

  • Abbie Bown

    (Public Health England)

  • Richard Vipond

    (Public Health England)

  • Alexander J. Mentzer

    (University of Oxford)

  • Julian C. Knight

    (University of Oxford
    John Radcliffe Hospital
    University of Oxford)

  • Andrew J. Kwok

    (University of Oxford)

  • Gavin R. Screaton

    (University of Oxford
    University of Oxford, John Radcliffe Hospital)

  • Juthathip Mongkolsapaya

    (University of Oxford
    University of Oxford
    Mahidol University)

  • Wanwisa Dejnirattisai

    (University of Oxford)

  • Piyada Supasa

    (University of Oxford)

  • Paul Klenerman

    (University of Oxford)

  • Christina Dold

    (University of Oxford
    University of Oxford)

  • J. Kenneth Baillie

    (University of Edinburgh)

  • Shona C. Moore

    (University of Liverpool)

  • Peter J. M. Openshaw

    (Imperial College London)

  • Malcolm G. Semple

    (University of Liverpool)

  • Lance C. W. Turtle

    (University of Liverpool)

  • Mark Ainsworth

    (Oxford University Hospitals NHS Foundation Trust)

  • Alice Allcock

    (University of Oxford)

  • Sally Beer

    (Oxford University Hospitals NHS Foundation Trust)

  • Sagida Bibi

    (University of Oxford)

  • Donal Skelly

    (Oxford University Hospitals NHS Foundation Trust)

  • Lizzy Stafford

    (Oxford University Hospitals NHS Foundation Trust)

  • Katie Jeffrey

    (Oxford University Hospitals NHS Foundation Trust)

  • Denise O’Donnell

    (Oxford University Hospitals NHS Foundation Trust)

  • Elizabeth Clutterbuck

    (University of Oxford)

  • Alexis Espinosa

    (Oxford University Hospitals NHS Foundation Trust)

  • Maria Mendoza

    (Oxford University Hospitals NHS Foundation Trust)

  • Dominique Georgiou

    (Oxford University Hospitals NHS Foundation Trust)

  • Teresa Lockett

    (Oxford University Hospitals NHS Foundation Trust)

  • Jose Martinez

    (Oxford University Hospitals NHS Foundation Trust)

  • Elena Perez

    (Oxford University Hospitals NHS Foundation Trust)

  • Veronica Gallardo Sanchez

    (Oxford University Hospitals NHS Foundation Trust)

  • Giuseppe Scozzafava

    (Oxford University Hospitals NHS Foundation Trust)

  • Alberto Sobrinodiaz

    (Oxford University Hospitals NHS Foundation Trust)

  • Hannah Thraves

    (Oxford University Hospitals NHS Foundation Trust)

  • Etienne Joly

    (University of Toulouse, CNRS)

Abstract

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

Suggested Citation

  • Alain Townsend & Pramila Rijal & Julie Xiao & Tiong Kit Tan & Kuan-Ying A. Huang & Lisa Schimanski & Jiandong Huo & Nimesh Gupta & Rolle Rahikainen & Philippa C. Matthews & Derrick Crook & Sarah Hoosd, 2021. "A haemagglutination test for rapid detection of antibodies to SARS-CoV-2," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22045-y
    DOI: 10.1038/s41467-021-22045-y
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    Cited by:

    1. Jia Wei & Philippa C. Matthews & Nicole Stoesser & Ian Diamond & Ruth Studley & Emma Rourke & Duncan Cook & John I. Bell & John N. Newton & Jeremy Farrar & Alison Howarth & Brian D. Marsden & Sarah Ho, 2022. "SARS-CoV-2 antibody trajectories after a single COVID-19 vaccination with and without prior infection," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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