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Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Author

Listed:
  • Anna Pascual-Reguant

    (Department of Rheumatology and Clinical Immunology
    Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Ralf Köhler

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Ronja Mothes

    (Department of Rheumatology and Clinical Immunology
    Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Sandy Bauherr

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Daniela C. Hernández

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Ralf Uecker

    (Department of Rheumatology and Clinical Immunology
    Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Karolin Holzwarth

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

  • Katja Kotsch

    (Charité - Universitätsmedizin Berlin, Department for General, Visceral and Vascular Surgery)

  • Maximilian Seidl

    (Medical Center—University of Freiburg
    Heinrich-Heine University and University Hospital of Düsseldorf)

  • Lars Philipsen

    (Institute of Molecular and Clinical Immunology, Medical Center)

  • Werner Müller

    (Miltenyi Biotec B.V. & Co. KG)

  • Chiara Romagnani

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
    Charité - Universitätsmedizin Berlin, Department of Gastroenterology, Infectiology and Rheumatology)

  • Raluca Niesner

    (Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
    Veterinary Medicine, Freie Universität Berlin)

  • Anja E. Hauser

    (Department of Rheumatology and Clinical Immunology
    Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute)

Abstract

Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.

Suggested Citation

  • Anna Pascual-Reguant & Ralf Köhler & Ronja Mothes & Sandy Bauherr & Daniela C. Hernández & Ralf Uecker & Karolin Holzwarth & Katja Kotsch & Maximilian Seidl & Lars Philipsen & Werner Müller & Chiara R, 2021. "Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21994-8
    DOI: 10.1038/s41467-021-21994-8
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    Cited by:

    1. Ronja Mothes & Anna Pascual-Reguant & Ralf Koehler & Juliane Liebeskind & Alina Liebheit & Sandy Bauherr & Lars Philipsen & Carsten Dittmayer & Michael Laue & Regina Manitius & Sefer Elezkurtaj & Pawe, 2023. "Distinct tissue niches direct lung immunopathology via CCL18 and CCL21 in severe COVID-19," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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