IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-21985-9.html
   My bibliography  Save this article

Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation

Author

Listed:
  • Ruifang Guan

    (National Institutes of Health)

  • Tengfei Lian

    (National Institutes of Health)

  • Bing-Rui Zhou

    (National Institutes of Health)

  • Emily He

    (Harvard University)

  • Carl Wu

    (Johns Hopkins University)

  • Martin Singleton

    (The Francis Crick Institute)

  • Yawen Bai

    (National Institutes of Health)

Abstract

Accurate chromosome segregation relies on the specific centromeric nucleosome–kinetochore interface. In budding yeast, the centromere CBF3 complex guides the deposition of CENP-A, an H3 variant, to form the centromeric nucleosome in a DNA sequence-dependent manner. Here, we determine the structures of the centromeric nucleosome containing the native CEN3 DNA and the CBF3core bound to the canonical nucleosome containing an engineered CEN3 DNA. The centromeric nucleosome core structure contains 115 base pair DNA including a CCG motif. The CBF3core specifically recognizes the nucleosomal CCG motif through the Gal4 domain while allosterically altering the DNA conformation. Cryo-EM, modeling, and mutational studies reveal that the CBF3core forms dynamic interactions with core histones H2B and CENP-A in the CEN3 nucleosome. Our results provide insights into the structure of the budding yeast centromeric nucleosome and the mechanism of its assembly, which have implications for analogous processes of human centromeric nucleosome formation.

Suggested Citation

  • Ruifang Guan & Tengfei Lian & Bing-Rui Zhou & Emily He & Carl Wu & Martin Singleton & Yawen Bai, 2021. "Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21985-9
    DOI: 10.1038/s41467-021-21985-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-21985-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-21985-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ksenia Smurova & Michela Damizia & Carmela Irene & Stefania Stancari & Giovanna Berto & Giulia Perticari & Maria Giuseppina Iacovella & Ilaria D’Ambrosio & Maria Giubettini & Réginald Philippe & Chiar, 2023. "Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21985-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.